Cerulein mRNA and peptide α-amidation activity in the skin of Xenopus laevis

Stimulation by norepinephrine

Eliot Spindel, Betty A. Eipper, Marya D. Zilberberg, Richard E. Mains, William W. Chin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The skin of Xenopus laevis, because of its high concentrations of amidated peptides, is an excellent model for the study of neuropeptide biosynthesis. In this paper we determined levels of the mRNAs which encode cerulin and characterized the enzymatic activity which likely produces the carboxyl terminal α-amide moiety of cerulein. This enzyme is stimulated by the addition of CuSO4 and ascorbate and requires the presence of molecular oxygen and is thus similar to the peptidyl-glycine α-amidating monooxygenase (PAM) described in mammals. Hybridization analysis showed that three cerulein mRNAs of different molecular sizes are present in varying proportions in the skin of individual frogs. Injection of norepinephrine into the dorsal lymph sac of Xenopus caused immediate secretion and depletion from skin of PAM activity but did not cause a comparable immediate decrease in skin levels of cerulein mRNA or total RNA. By 2 weeks after norepinephrine injection, cerulein mRNA levels increased to fourfold over control levels. By 4 weeks, skin PAM activity was almost restored to control levels.

Original languageEnglish (US)
Pages (from-to)67-76
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume67
Issue number1
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Ceruletide
Xenopus laevis
norepinephrine
skin (animal)
Norepinephrine
peptides
Messenger RNA
Skin
Peptides
Mixed Function Oxygenases
glycine (amino acid)
Glycine
injection
Injections
copper sulfate
lymph
neuropeptides
Xenopus
Lymph
amides

ASJC Scopus subject areas

  • Endocrinology

Cite this

Cerulein mRNA and peptide α-amidation activity in the skin of Xenopus laevis : Stimulation by norepinephrine. / Spindel, Eliot; Eipper, Betty A.; Zilberberg, Marya D.; Mains, Richard E.; Chin, William W.

In: General and Comparative Endocrinology, Vol. 67, No. 1, 1987, p. 67-76.

Research output: Contribution to journalArticle

Spindel, Eliot ; Eipper, Betty A. ; Zilberberg, Marya D. ; Mains, Richard E. ; Chin, William W. / Cerulein mRNA and peptide α-amidation activity in the skin of Xenopus laevis : Stimulation by norepinephrine. In: General and Comparative Endocrinology. 1987 ; Vol. 67, No. 1. pp. 67-76.
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