TY - JOUR
T1 - Cerebellum plus locus coeruleus in tissue culture II
T2 - Development and metabolism of catecholamines
AU - Woodward, W. R.
AU - Seil, F. J.
AU - Hammerstad, J. P.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1987
Y1 - 1987
N2 - In contrast to locus coeruleus neurons in vivo, dopamine was the predominant catecholamine synthesized, stored, and released by neonatal mouse locus coeruleus cultures which included target cerebellar tissue, and norepinephrine was present in these cultures only at very low levels. Developmentally, norepinephrine increased slightly in the explants during the first 4 days in vitro and declined thereafter to barely detectable levels, whereas dopamine began to rise after 4 days and reached maximal levels by 7 days. Dopamine β‐hydroxylase was present in these cultures throughout maturation. These results suggest that the high ratio of dopamine to norepinephrine in locus coeruleus cultures cannot be attributed to the absence of appropriate target tissue or to a lack of the enzyme, dopamine β‐hydroxylase.
AB - In contrast to locus coeruleus neurons in vivo, dopamine was the predominant catecholamine synthesized, stored, and released by neonatal mouse locus coeruleus cultures which included target cerebellar tissue, and norepinephrine was present in these cultures only at very low levels. Developmentally, norepinephrine increased slightly in the explants during the first 4 days in vitro and declined thereafter to barely detectable levels, whereas dopamine began to rise after 4 days and reached maximal levels by 7 days. Dopamine β‐hydroxylase was present in these cultures throughout maturation. These results suggest that the high ratio of dopamine to norepinephrine in locus coeruleus cultures cannot be attributed to the absence of appropriate target tissue or to a lack of the enzyme, dopamine β‐hydroxylase.
KW - coeruleocerebellar cultures
KW - dopamine
KW - dopamine β‐hydroxylase
KW - neuron‐target interaction
KW - norepinephrine
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U2 - 10.1002/jnr.490170214
DO - 10.1002/jnr.490170214
M3 - Article
C2 - 3586071
AN - SCOPUS:0023157159
VL - 17
SP - 184
EP - 188
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 2
ER -