Centromeric index measurement by slit-scan flow cytometry.

J. N. Lucas, Joe Gray, D. C. Peters, M. A. Van Dilla

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We report here the application of slit-scan flow cytometry (SSFCM) in the classification of muntjac, Chinese hamster, and human chromosomes according to centromeric index (CI) and total fluorescence. Chromosomes were isolated from mitotic cells, stained with propidium iodide and processed through the SSFCM where fluorescence profiles were measured. The centromere for each profile was taken as the point of maximum difference between the measured profile and a standard profile having no centromeric dip. The areas under the profile on either side of the centromere were then calculated and the CI was calculated as the ratio of the larger area to the total area under the profile. Relative DNA contents for each chromosome were taken to be proportional to the total fluorescence. Mean CI's for muntjac chromosomes 1, 2, and X + 3 were 0.52, 0.88, and 0.73, respectively; CI's for Chinese hamster M3-1 chromosomes 1, 2, 5, 8, and M2 were 0.53, 0.55, 0.57, 0.77, and 0.86, respectively; and average CI's for chromosome groups 4 + t (X;5), 6 + 7 + Y, 9 + M1, and 10 + 11 were 0.56, 0.82, 0.58, and 0.60, respectively. These results were, on average, within 4.4% of CI measurements made by image cytometry. CI's measured for human chromosomes 9 through 12, were, on average, within 2.0% of those made by image cytometry.

Original languageEnglish (US)
Pages (from-to)109-116
Number of pages8
JournalCytometry
Volume4
Issue number2
StatePublished - Sep 1983
Externally publishedYes

Fingerprint

Muntjacs
Image Cytometry
Flow Cytometry
Chromosomes, Human, Pair 2
Centromere
Fluorescence
Chromosomes, Human, Pair 1
Human Chromosomes
Cricetulus
Chromosomes
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 4
Propidium
DNA

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Endocrinology
  • Hematology
  • Pathology and Forensic Medicine

Cite this

Lucas, J. N., Gray, J., Peters, D. C., & Van Dilla, M. A. (1983). Centromeric index measurement by slit-scan flow cytometry. Cytometry, 4(2), 109-116.

Centromeric index measurement by slit-scan flow cytometry. / Lucas, J. N.; Gray, Joe; Peters, D. C.; Van Dilla, M. A.

In: Cytometry, Vol. 4, No. 2, 09.1983, p. 109-116.

Research output: Contribution to journalArticle

Lucas, JN, Gray, J, Peters, DC & Van Dilla, MA 1983, 'Centromeric index measurement by slit-scan flow cytometry.', Cytometry, vol. 4, no. 2, pp. 109-116.
Lucas JN, Gray J, Peters DC, Van Dilla MA. Centromeric index measurement by slit-scan flow cytometry. Cytometry. 1983 Sep;4(2):109-116.
Lucas, J. N. ; Gray, Joe ; Peters, D. C. ; Van Dilla, M. A. / Centromeric index measurement by slit-scan flow cytometry. In: Cytometry. 1983 ; Vol. 4, No. 2. pp. 109-116.
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