Centella asiatica Attenuates Mitochondrial Dysfunction and Oxidative Stress in Aβ-Exposed Hippocampal Neurons

Nora E. Gray, Jonathan A. Zweig, Donald G. Matthews, Maya Caruso, Joseph Quinn, Amala Soumyanath

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Centella asiatica has been used for centuries to enhance memory. We have previously shown that a water extract of Centella asiatica (CAW) protects against the deleterious effects of amyloid-β (Aβ) in neuroblastoma cells and attenuates Aβ-induced cognitive deficits in mice. Yet, the neuroprotective mechanism of CAW has yet to be thoroughly explored in neurons from these animals. This study investigates the effects of CAW on neuronal metabolism and oxidative stress in isolated Aβ-expressing neurons. Hippocampal neurons from amyloid precursor protein overexpressing Tg2576 mice and wild-type (WT) littermates were treated with CAW. In both genotypes, CAW increased the expression of antioxidant response genes which attenuated the Aβ-induced elevations in reactive oxygen species (ROS) and lipid peroxidation in Tg2576 neurons. CAW also improved mitochondrial function in both genotypes and increased the expression of electron transport chain enzymes and mitochondrial labeling, suggesting an increase in mitochondrial content. These data show that CAW protects against mitochondrial dysfunction and oxidative stress in Aβ-exposed hippocampal neurons which could contribute to the beneficial effects of the extract observed in vivo. Since CAW also improved mitochondrial function in the absence of Aβ, these results suggest a broader utility for other conditions where neuronal mitochondrial dysfunction occurs.

Original languageEnglish (US)
Article number7023091
JournalOxidative Medicine and Cellular Longevity
Volume2017
DOIs
StatePublished - 2017

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Centella
Oxidative stress
Neurons
Oxidative Stress
Genotype
Amyloid beta-Protein Precursor
Electron Transport
Neuroblastoma
Amyloid
Metabolism
Labeling
Lipid Peroxidation
Reactive Oxygen Species
Animals
Antioxidants
Genes
Lipids
Data storage equipment
Water
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Cell Biology

Cite this

Centella asiatica Attenuates Mitochondrial Dysfunction and Oxidative Stress in Aβ-Exposed Hippocampal Neurons. / Gray, Nora E.; Zweig, Jonathan A.; Matthews, Donald G.; Caruso, Maya; Quinn, Joseph; Soumyanath, Amala.

In: Oxidative Medicine and Cellular Longevity, Vol. 2017, 7023091, 2017.

Research output: Contribution to journalArticle

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