Cellular retinol- and retinoic acid-binding proteins in transformed mammalian cells

J. C. Saari, S. Futterman, G. W. Stubbs, J. T. Heffernan, L. Bredberg, D. Y. Chan, D. M. Albert

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Extracts prepared from several lines of transformed cells were examined for the presence of cellular binding proteins specific for retinoids. Extracts of human retinoblastoma cell line WERI-Rb1 contained a cellular binding protein specific for retinoic acid, whereas extracts of human retinoblastoma cell line Y-79 contained cellular binding proteins for both retinol and retinoic acid. Upon purification, the latter two binding proteins proved to have properties similar to those of the corresponding proteins obtained from bovine retina. Smaller amounts of these binding proteins were detected in extracts of undifferentiated and differentiated neuroblastoma and McCoy cells. HeLa and rat glioma cells had no detectable amount of binding proteins. The 11-cis-retinal-binding protein, present in extracts of human, rat, and bovine retina, was not found in any of the cell lines examined.

Original languageEnglish (US)
Pages (from-to)988-992
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume17
Issue number10
StatePublished - Dec 11 1978

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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