Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients

C. A. Wiley, R. D. Schrier, Jay Nelson, P. W. Lampert, M. B. Oldstone

Research output: Contribution to journalArticle

997 Citations (Scopus)

Abstract

Dysfunction of the central nervous system (CNS) is a prominent feature of the acquired immune deficiency syndrome (AIDS). Many of these patients have a subacute encephalitis consistent with a viral infection of the CNS. We studied the brains of 12 AIDS patients using in situ hybridization to identify human immunodeficiency virus [HIV, referred to by others as human T-cell lymphotropic virus type III (HTLV-III), lymphadenopathy-associated virus (LAV), AIDS-associated retrovirus (ARV)] nucleic acid sequences and immunocytochemistry to identify viral and cellular proteins. Nine patients had significant HIV infection in the CNS. In all examined brains, the white matter was more severely involved than the grey matter. In most cases the infection was restricted to capillary endothelial cells, mononuclear inflammatory cells, and giant cells. In a single case with severe CNS involvement, a low-level infection was seen in some astrocytes and neurons. These results suggest that CNS dysfunction is due to indirect effects rather than neuronal or glial infection.

Original languageEnglish (US)
Pages (from-to)7089-7093
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number18
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Virus Diseases
Acquired Immunodeficiency Syndrome
Central Nervous System
HIV
Brain
Central Nervous System Viral Diseases
Infection
Viral Proteins
Encephalitis
Giant Cells
Retroviridae
Neuroglia
Astrocytes
Nucleic Acids
HIV Infections
In Situ Hybridization
Endothelial Cells
Immunohistochemistry
Neurons

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients. / Wiley, C. A.; Schrier, R. D.; Nelson, Jay; Lampert, P. W.; Oldstone, M. B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 18, 1986, p. 7089-7093.

Research output: Contribution to journalArticle

@article{74d7507239254b2d8ebd3c64b12c7891,
title = "Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients",
abstract = "Dysfunction of the central nervous system (CNS) is a prominent feature of the acquired immune deficiency syndrome (AIDS). Many of these patients have a subacute encephalitis consistent with a viral infection of the CNS. We studied the brains of 12 AIDS patients using in situ hybridization to identify human immunodeficiency virus [HIV, referred to by others as human T-cell lymphotropic virus type III (HTLV-III), lymphadenopathy-associated virus (LAV), AIDS-associated retrovirus (ARV)] nucleic acid sequences and immunocytochemistry to identify viral and cellular proteins. Nine patients had significant HIV infection in the CNS. In all examined brains, the white matter was more severely involved than the grey matter. In most cases the infection was restricted to capillary endothelial cells, mononuclear inflammatory cells, and giant cells. In a single case with severe CNS involvement, a low-level infection was seen in some astrocytes and neurons. These results suggest that CNS dysfunction is due to indirect effects rather than neuronal or glial infection.",
author = "Wiley, {C. A.} and Schrier, {R. D.} and Jay Nelson and Lampert, {P. W.} and Oldstone, {M. B.}",
year = "1986",
doi = "10.1073/pnas.83.18.7089",
language = "English (US)",
volume = "83",
pages = "7089--7093",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "18",

}

TY - JOUR

T1 - Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients

AU - Wiley, C. A.

AU - Schrier, R. D.

AU - Nelson, Jay

AU - Lampert, P. W.

AU - Oldstone, M. B.

PY - 1986

Y1 - 1986

N2 - Dysfunction of the central nervous system (CNS) is a prominent feature of the acquired immune deficiency syndrome (AIDS). Many of these patients have a subacute encephalitis consistent with a viral infection of the CNS. We studied the brains of 12 AIDS patients using in situ hybridization to identify human immunodeficiency virus [HIV, referred to by others as human T-cell lymphotropic virus type III (HTLV-III), lymphadenopathy-associated virus (LAV), AIDS-associated retrovirus (ARV)] nucleic acid sequences and immunocytochemistry to identify viral and cellular proteins. Nine patients had significant HIV infection in the CNS. In all examined brains, the white matter was more severely involved than the grey matter. In most cases the infection was restricted to capillary endothelial cells, mononuclear inflammatory cells, and giant cells. In a single case with severe CNS involvement, a low-level infection was seen in some astrocytes and neurons. These results suggest that CNS dysfunction is due to indirect effects rather than neuronal or glial infection.

AB - Dysfunction of the central nervous system (CNS) is a prominent feature of the acquired immune deficiency syndrome (AIDS). Many of these patients have a subacute encephalitis consistent with a viral infection of the CNS. We studied the brains of 12 AIDS patients using in situ hybridization to identify human immunodeficiency virus [HIV, referred to by others as human T-cell lymphotropic virus type III (HTLV-III), lymphadenopathy-associated virus (LAV), AIDS-associated retrovirus (ARV)] nucleic acid sequences and immunocytochemistry to identify viral and cellular proteins. Nine patients had significant HIV infection in the CNS. In all examined brains, the white matter was more severely involved than the grey matter. In most cases the infection was restricted to capillary endothelial cells, mononuclear inflammatory cells, and giant cells. In a single case with severe CNS involvement, a low-level infection was seen in some astrocytes and neurons. These results suggest that CNS dysfunction is due to indirect effects rather than neuronal or glial infection.

UR - http://www.scopus.com/inward/record.url?scp=0000969952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000969952&partnerID=8YFLogxK

U2 - 10.1073/pnas.83.18.7089

DO - 10.1073/pnas.83.18.7089

M3 - Article

VL - 83

SP - 7089

EP - 7093

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 18

ER -