Cell type-specific transcriptomic analysis by thiouracil tagging in zebrafish

T. Erickson, Teresa Nicolson

Research output: Contribution to journalArticle

Abstract

Transcriptomic studies are important tools for understanding the development and function of the different cell types that make up complex tissues. Zebrafish (Danio rerio) is a valuable organism for modeling key aspects of vertebrate development, cell biology, and human disease. However, the small size of individual larvae and relative scarcity of certain cell types in zebrafish can hamper efforts to collect enough pure material for cell type-specific transcriptomic studies. Thus, there is a need in the zebrafish field for spatially and temporally resolved gene expression assays. This chapter will discuss the general principles behind the TU-tagging method to isolate cell type-specific RNAs and provide guidance in designing and executing TU-tagging experiments in zebrafish.

Original languageEnglish (US)
JournalMethods in Cell Biology
DOIs
StateAccepted/In press - 2016

Fingerprint

Thiouracil
Zebrafish
Larva
Cell Biology
Vertebrates
RNA
Gene Expression

Keywords

  • Gene expression
  • RNA
  • Thiouracil
  • Transcriptomics
  • TU-Tagging
  • UPRT
  • Zebrafish

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell type-specific transcriptomic analysis by thiouracil tagging in zebrafish. / Erickson, T.; Nicolson, Teresa.

In: Methods in Cell Biology, 2016.

Research output: Contribution to journalArticle

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