TY - JOUR
T1 - Cell surface receptors for murine leukemia viruses
T2 - Two assays and their implications
AU - Handelin, Barbara L.
AU - Kabat, David
N1 - Funding Information:
We thank Ethel Polonoff for excellent technical assistance. B.L.H. is a graduate student in the Department of Medical Genetics and is a Tartar Trust Fellow. This research was supported by Grant PCM 8218398f rom the National Science Foundation.
PY - 1985/1/15
Y1 - 1985/1/15
N2 - Two assays were developed for identifying individual cells which bear murine leukemia virus receptors: an erythrocyte rosette assay for ecotropic receptors, and an efficient immune cytotoxic assay for cells with ecotropic or amphotropic receptors. Both assays indicate that ecotropic MuLV adsorbed to its cell surface receptor only slowly becomes internalized. Furthermore, attempts to isolate murine fibroblast variants lacking these ecotropic MuLV receptors were unsuccessful, suggesting either that mutations in the receptor gene are rare (less than 10' per generation) or that the receptor is required for cell viability. These assays are rapid and can be used to identify receptor-bearing cells in mixed populations, a prerequisite for molecular genetic studies.
AB - Two assays were developed for identifying individual cells which bear murine leukemia virus receptors: an erythrocyte rosette assay for ecotropic receptors, and an efficient immune cytotoxic assay for cells with ecotropic or amphotropic receptors. Both assays indicate that ecotropic MuLV adsorbed to its cell surface receptor only slowly becomes internalized. Furthermore, attempts to isolate murine fibroblast variants lacking these ecotropic MuLV receptors were unsuccessful, suggesting either that mutations in the receptor gene are rare (less than 10' per generation) or that the receptor is required for cell viability. These assays are rapid and can be used to identify receptor-bearing cells in mixed populations, a prerequisite for molecular genetic studies.
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U2 - 10.1016/0042-6822(85)90458-1
DO - 10.1016/0042-6822(85)90458-1
M3 - Article
C2 - 3966298
AN - SCOPUS:0021989451
SN - 0042-6822
VL - 140
SP - 183
EP - 187
JO - Virology
JF - Virology
IS - 1
ER -