Cell surface receptors for murine leukemia viruses

Two assays and their implications

Barbara L. Handelin, David Kabat

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two assays were developed for identifying individual cells which bear murine leukemia virus receptors: an erythrocyte rosette assay for ecotropic receptors, and an efficient immune cytotoxic assay for cells with ecotropic or amphotropic receptors. Both assays indicate that ecotropic MuLV adsorbed to its cell surface receptor only slowly becomes internalized. Furthermore, attempts to isolate murine fibroblast variants lacking these ecotropic MuLV receptors were unsuccessful, suggesting either that mutations in the receptor gene are rare (less than 10' per generation) or that the receptor is required for cell viability. These assays are rapid and can be used to identify receptor-bearing cells in mixed populations, a prerequisite for molecular genetic studies.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalVirology
Volume140
Issue number1
DOIs
StatePublished - Jan 15 1985

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Murine Leukemia Viruses
Cell Surface Receptors
Virus Receptors
Molecular Biology
Cell Survival
Fibroblasts
Erythrocytes
Mutation
Population
Genes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Cell surface receptors for murine leukemia viruses : Two assays and their implications. / Handelin, Barbara L.; Kabat, David.

In: Virology, Vol. 140, No. 1, 15.01.1985, p. 183-187.

Research output: Contribution to journalArticle

Handelin, Barbara L. ; Kabat, David. / Cell surface receptors for murine leukemia viruses : Two assays and their implications. In: Virology. 1985 ; Vol. 140, No. 1. pp. 183-187.
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