Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter

Hao Wang, Michael P. Kavanaugh, R. Alan North, David Kabat

Research output: Contribution to journalArticle

326 Citations (Scopus)

Abstract

THE complementary DNA sequence encoding the cell-surface receptor for ecotropic host-range murine retroviruses (ecoR) shows that it contains 622 amino acids and 14 Hydrophobic potentially membrane-spanning sequences1. Because this receptor occurs on many or all murine cells2 and is probably essential for viability of cultured fibroblasts3, its normal function might be to transport an essential metabolite. We expressed ecoR in Xenopus laevis oocytes by injecting RNA transcribed from the cloned cDNA. These oocytes specifically bound the gp70 envelope glycoprotein from an ecotropic murine leukaemia virus. An inward current was recorded electrophysiologically when a mixture of amino-acids was applied: this resulted from a stereoselective, saturable uptake of lysine, arginine and ornithine; it was independent of sodium and not substantially altered by gp70. Cysteine and homoserine were also taken up, but sodium was necessary for their transport. These properties of ecoR correspond to those of the y+ amino-acid transporter4-6. Our results demonstrate the subversion of a ubiquitous cell membrane protein, in this case a basic amino acid transporter, for use as a retroviral receptor.

Original languageEnglish (US)
Pages (from-to)729-731
Number of pages3
JournalNature
Volume352
Issue number6337
StatePublished - Aug 22 1991

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Amino Acid Transport Systems
Basic Amino Acids
Cell Surface Receptors
Retroviridae
Amino Acids
Oocytes
Complementary DNA
Sodium
Homoserine
Murine Leukemia Viruses
Ornithine
Host Specificity
Xenopus laevis
Lysine
Cysteine
Arginine
Glycoproteins
Membrane Proteins
RNA
Membranes

ASJC Scopus subject areas

  • General

Cite this

Wang, H., Kavanaugh, M. P., North, R. A., & Kabat, D. (1991). Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. Nature, 352(6337), 729-731.

Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. / Wang, Hao; Kavanaugh, Michael P.; North, R. Alan; Kabat, David.

In: Nature, Vol. 352, No. 6337, 22.08.1991, p. 729-731.

Research output: Contribution to journalArticle

Wang, H, Kavanaugh, MP, North, RA & Kabat, D 1991, 'Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter', Nature, vol. 352, no. 6337, pp. 729-731.
Wang H, Kavanaugh MP, North RA, Kabat D. Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. Nature. 1991 Aug 22;352(6337):729-731.
Wang, Hao ; Kavanaugh, Michael P. ; North, R. Alan ; Kabat, David. / Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. In: Nature. 1991 ; Vol. 352, No. 6337. pp. 729-731.
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