Cell surface AMPA receptors in the rat nucleus accumbens increase during cocaine withdrawal but internalize after cocaine challenge in association with altered activation of mitogen-activated protein kinases

Amy C. Boudreau, Jeremy M. Reimers, Michael Milovanovic, Marina Wolf

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

Although some studies report increased responsiveness of nucleus accumbens (NAc) AMPA receptors (AMPARs) after withdrawal from repeated cocaine treatment, others report decreased responsiveness after withdrawal plus cocaine challenge. Here we examine this apparent contradiction by quantifying cell surface and intracellular AMPAR subunits in the NAc before and after a challenge injection in behaviorally sensitized rats. Because MAPKs (mitogen-activated protein kinases) regulate AMPAR trafficking and are implicated in addiction, we also evaluated phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. Glutamate receptor 1 (GluR1) and GluR2 surface/intracellular (S/I) ratios were increased after 14 d of withdrawal in sensitized rats but were decreased 24 h after challenge with cocaine (which elicited a sensitized locomotor response) or saline (which elicited conditioned locomotion). These findings suggested redistribution of GluR1/2-containing receptors, a possibility supported by immunoprecipitation experiments indicating that most AMPARs in the NAc are GluR1/2 or GluR2/3, with few homomeric GluR1 or GluR1/3 receptors. In sensitized rats, ERK phosphorylation in the NAc increased during withdrawal and normalized after cocaine challenge. JNK phosphorylation also increased after withdrawal, but after cocaine challenge, it was inversely related to GluR1 and GluR2 S/I ratios. After saline challenge, p38 phosphorylation was increased. In summary, surface expression of GluR1/2-containing AMPARs increased in the NAc of sensitized rats, but AMPARs internalized after a single reexposure to cocaine or cocaine-related cues. ERK phosphorylation paralleled AMPAR surface expression. Although JNK results were complex, JNK and p38 may be involved in AMPAR internalization after cocaine or saline challenge, respectively.

Original languageEnglish (US)
Pages (from-to)10621-10635
Number of pages15
JournalJournal of Neuroscience
Volume27
Issue number39
DOIs
StatePublished - Sep 26 2007
Externally publishedYes

Fingerprint

AMPA Receptors
Nucleus Accumbens
Cell Surface Receptors
Mitogen-Activated Protein Kinases
Cocaine
Glutamate Receptors
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Phosphotransferases
JNK Mitogen-Activated Protein Kinases
Locomotion
Immunoprecipitation
Cues
Injections

Keywords

  • Addiction
  • AMPA receptor
  • Cocaine
  • Nucleus accumbens
  • Rat
  • Sensitization
  • Trafficking

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cell surface AMPA receptors in the rat nucleus accumbens increase during cocaine withdrawal but internalize after cocaine challenge in association with altered activation of mitogen-activated protein kinases. / Boudreau, Amy C.; Reimers, Jeremy M.; Milovanovic, Michael; Wolf, Marina.

In: Journal of Neuroscience, Vol. 27, No. 39, 26.09.2007, p. 10621-10635.

Research output: Contribution to journalArticle

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