Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells.

K. A. Sevarino, R. Felix, C. M. Banks, M. J. Low, M. R. Montminy, Gail Mandel, Richard Goodman

Research output: Contribution to journalArticle

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Abstract

We have previously found that preprosomatostatin is processed accurately to both somatostatin-14 and somatostatin-28 in pituitary gonadotrophs of transgenic mice. The foreign somatostatin peptides have been shown to enter the regulated secretory pathway of these cells. To determine whether accurate preprosomatostatin processing can occur in any neuroendocrine cell, we introduced preprosomatostatin cDNA expression vectors into several different neuroendocrine cell lines. We found that prosomatostatin was cleaved efficiently to somatostatin-14 and somatostatin-28 in RIN 5F and AtT20 cells, but not in GH4 or PC12 cells. The ability of a particular cell type to process prosomatostatin did not correlate with cellular storage capacity and was independent of the level of biosynthesis of the precursor. These data suggest that prosomatostatin processing requires specific pathways which are present in some neuroendocrine cells, but not in others.

Original languageEnglish (US)
Pages (from-to)4987-4993
Number of pages7
JournalJournal of Biological Chemistry
Volume262
Issue number11
StatePublished - Apr 15 1987
Externally publishedYes

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Neuroendocrine Cells
Somatostatin-28
Somatostatin
Cultured Cells
Processing
Gonadotrophs
Secretory Pathway
PC12 Cells
Biosynthesis
Transgenic Mice
Complementary DNA
Cells
Cell Line
Peptides
prosomatostatin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sevarino, K. A., Felix, R., Banks, C. M., Low, M. J., Montminy, M. R., Mandel, G., & Goodman, R. (1987). Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells. Journal of Biological Chemistry, 262(11), 4987-4993.

Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells. / Sevarino, K. A.; Felix, R.; Banks, C. M.; Low, M. J.; Montminy, M. R.; Mandel, Gail; Goodman, Richard.

In: Journal of Biological Chemistry, Vol. 262, No. 11, 15.04.1987, p. 4987-4993.

Research output: Contribution to journalArticle

Sevarino, KA, Felix, R, Banks, CM, Low, MJ, Montminy, MR, Mandel, G & Goodman, R 1987, 'Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells.', Journal of Biological Chemistry, vol. 262, no. 11, pp. 4987-4993.
Sevarino KA, Felix R, Banks CM, Low MJ, Montminy MR, Mandel G et al. Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells. Journal of Biological Chemistry. 1987 Apr 15;262(11):4987-4993.
Sevarino, K. A. ; Felix, R. ; Banks, C. M. ; Low, M. J. ; Montminy, M. R. ; Mandel, Gail ; Goodman, Richard. / Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 11. pp. 4987-4993.
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