Cell-selective metabolic labeling of proteins

John T. Ngo, Julie A. Champion, Alborz Mahdavi, I. Caglar Tanrikulu, Kimberly Beatty, Rebecca E. Connor, Tae Hyeon Yoo, Daniela C. Dieterich, Erin M. Schuman, David A. Tirrell

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.

Original languageEnglish (US)
Pages (from-to)715-717
Number of pages3
JournalNature Chemical Biology
Volume5
Issue number10
DOIs
StatePublished - Oct 2009
Externally publishedYes

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Ligases
Methionine-tRNA Ligase
Proteins
Complex Mixtures
Fluorescent Dyes
Methionine
azidonorleucine

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Ngo, J. T., Champion, J. A., Mahdavi, A., Tanrikulu, I. C., Beatty, K., Connor, R. E., ... Tirrell, D. A. (2009). Cell-selective metabolic labeling of proteins. Nature Chemical Biology, 5(10), 715-717. https://doi.org/10.1038/nchembio.200

Cell-selective metabolic labeling of proteins. / Ngo, John T.; Champion, Julie A.; Mahdavi, Alborz; Tanrikulu, I. Caglar; Beatty, Kimberly; Connor, Rebecca E.; Yoo, Tae Hyeon; Dieterich, Daniela C.; Schuman, Erin M.; Tirrell, David A.

In: Nature Chemical Biology, Vol. 5, No. 10, 10.2009, p. 715-717.

Research output: Contribution to journalArticle

Ngo, JT, Champion, JA, Mahdavi, A, Tanrikulu, IC, Beatty, K, Connor, RE, Yoo, TH, Dieterich, DC, Schuman, EM & Tirrell, DA 2009, 'Cell-selective metabolic labeling of proteins', Nature Chemical Biology, vol. 5, no. 10, pp. 715-717. https://doi.org/10.1038/nchembio.200
Ngo JT, Champion JA, Mahdavi A, Tanrikulu IC, Beatty K, Connor RE et al. Cell-selective metabolic labeling of proteins. Nature Chemical Biology. 2009 Oct;5(10):715-717. https://doi.org/10.1038/nchembio.200
Ngo, John T. ; Champion, Julie A. ; Mahdavi, Alborz ; Tanrikulu, I. Caglar ; Beatty, Kimberly ; Connor, Rebecca E. ; Yoo, Tae Hyeon ; Dieterich, Daniela C. ; Schuman, Erin M. ; Tirrell, David A. / Cell-selective metabolic labeling of proteins. In: Nature Chemical Biology. 2009 ; Vol. 5, No. 10. pp. 715-717.
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