Cell penetrating recombinant Foxp3 protein enhances Treg function and ameliorates arthritis

Kentaro Yomogida, Shili Wu, Bobby Baravati, Camilo Avendano, Tom Caldwell, Brian Maniaci, Yong Zhu, Cong Qiu Chu

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Foxp3 is the master transcription factor for T regulatory (Treg) cell differentiation and function. This study aimed to test the therapeutic potential of cell penetrating recombinant Foxp3 protein in arthritis. Recombinant Foxp3 protein was fused to a cell penetrating polyarginine (Foxp3-11R) tag to facilitate intracellular transduction. In vitro Foxp3-11R treated CD4+ T cells showed a 50% increase in suppressive function compared with control protein treated cells. Severity of arthritis in Foxp3-11R treated mice was significantly reduced compared with those treated with a control protein. CD4+ T cells of lymph nodes and spleen from Foxp3-11R treated mice showed increased levels of Foxp3 expression compared with those of a control protein treated. These results demonstrated that Foxp3-11R can enhance T cell suppressive function and ameliorate experimental arthritis and suggest that cell penetrating recombinant Foxp3 is a potentially useful agent in therapy of arthritis.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume434
Issue number2
DOIs
StatePublished - May 3 2013

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Keywords

  • Arthritis
  • Foxp3
  • T regulatory cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yomogida, K., Wu, S., Baravati, B., Avendano, C., Caldwell, T., Maniaci, B., Zhu, Y., & Chu, C. Q. (2013). Cell penetrating recombinant Foxp3 protein enhances Treg function and ameliorates arthritis. Biochemical and Biophysical Research Communications, 434(2), 263-267. https://doi.org/10.1016/j.bbrc.2013.02.114