Cell-matrix adhesions on poly(vinyl alcohol) hydrogels

Miles B. Zajaczkowski, Edna Cukierman, Catherine Galbraith, Kenneth M. Yamada

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Cell-matrix adhesions regulate cell morphology, intracellular signaling, gene expression, and phenotype. Understanding how different methods of attaching matrix proteins to substrates affect the molecular arrangement of these adhesions offers the possibility of controlling cell function and architecture. The goal of this study was to visualize and quantify the cell-matrix adhesions formed by human fibroblasts on the matrix protein fibronectin covalently attached to poly(vinyl) alcohol (PVA) hydrogels. These adhesions were then compared with the cell adhesions formed in routine cell culture on fibronectin noncovalently coated onto glass coverslips or those formed on fibronectin covalently immobilized onto glass coverslips. Cell adhesions were characterized by immunofluorescence confocal microscopy utilizing paxillin as a marker for focal adhesions and α5 integrin as a marker for fibrillar adhesions. As expected, distinct focal and fibrillar adhesions were observed in routine cell culture on coverslips coated noncovalently with fibronectin. Cells cultured on fibronectin covalently linked to PVA demonstrated diminished spatial separation of paxillin and α5 integrin, accompanied by a reduction in fibrillar adhesions and fibronectin fibrillogenesis. Cells on fibronectin covalently immobilized on glass displayed the strongest marker colocalization and the most complete loss of fibrillar adhesions and lack of fibrillogenesis. These results indicate that fibronectinconjugated PVA promotes the formation of cell adhesion structures intermediate in composition between those formed on noncovalently attached and covalently immobilized fibronectin. Furthermore, they imply that bioactive polymers can selectively induce specific cell-matrix adhesions, a characteristic that may have consequences in various tissue-engineering applications.

Original languageEnglish (US)
Pages (from-to)525-533
Number of pages9
JournalTissue Engineering
Volume9
Issue number3
DOIs
StatePublished - Jun 2003
Externally publishedYes

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Cell-Matrix Junctions
Hydrogels
Fibronectins
Alcohols
Adhesion
Cell adhesion
Cell Adhesion
Paxillin
Glass
Focal Adhesions
Integrins
Cell culture
Cell Culture Techniques
Proteins
Tissue Engineering
Confocal microscopy
Fluorescence Microscopy
Confocal Microscopy
Fibroblasts
Tissue engineering

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

Cite this

Cell-matrix adhesions on poly(vinyl alcohol) hydrogels. / Zajaczkowski, Miles B.; Cukierman, Edna; Galbraith, Catherine; Yamada, Kenneth M.

In: Tissue Engineering, Vol. 9, No. 3, 06.2003, p. 525-533.

Research output: Contribution to journalArticle

Zajaczkowski, Miles B. ; Cukierman, Edna ; Galbraith, Catherine ; Yamada, Kenneth M. / Cell-matrix adhesions on poly(vinyl alcohol) hydrogels. In: Tissue Engineering. 2003 ; Vol. 9, No. 3. pp. 525-533.
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