Cell growth-dependent coordination of lipid signaling and glycosylation is mediated by interactions between Sac1p and Dpm1p

Frank Faulhammer, Gerlinde Konrad, Ben Brankatschk, Sabina Tahirovic, Andreas Knödler, Peter Mayinger

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The integral membrane lipid phosphatase Sac1p regulates local pools of phosphatidylinositol-4-phosphate (PtdIns(4)P) at endoplasmic reticulum (ER) and Golgi membranes. PtdIns(4)P is important for Golgi trafficking, yet the significance of PtdIns(4)P for ER function is unknown. It also remains unknown how localization of Sac1p to distinct organellar membranes is mediated. Here, we show that a COOH-terminal region in yeast Sac1p is crucial for ER targeting by directly interacting with dolicholphosphate mannose synthase Dpm1p. The interaction with Dpm1p persists during exponential cell division but is rapidly abolished when cell growth slows because of nutrient limitation, causing translocation of Sac1p to Golgi membranes. Cell growth-dependent shuttling of Sac1p between the ER and the Golgi is important for reciprocal control of PtdIns(4)P levels at these organelles. The fraction of Sac1p resident at the ER is also required for efficient dolichol oligosaccharide biosynthesis. Thus, the lipid phosphatase Sac1p may be a key regulator, coordinating the secretory capacity of ER and Golgi membranes in response to growth conditions.

Original languageEnglish (US)
Pages (from-to)185-191
Number of pages7
JournalJournal of Cell Biology
Volume168
Issue number2
DOIs
StatePublished - Jan 17 2005

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Glycosylation
Endoplasmic Reticulum
Lipids
Growth
Membranes
Phosphoric Monoester Hydrolases
Dolichol Monophosphate Mannose
Dolichol
Membrane Lipids
Oligosaccharides
Organelles
Cell Division
Yeasts
Food
phosphatidylinositol 4-phosphate

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell growth-dependent coordination of lipid signaling and glycosylation is mediated by interactions between Sac1p and Dpm1p. / Faulhammer, Frank; Konrad, Gerlinde; Brankatschk, Ben; Tahirovic, Sabina; Knödler, Andreas; Mayinger, Peter.

In: Journal of Cell Biology, Vol. 168, No. 2, 17.01.2005, p. 185-191.

Research output: Contribution to journalArticle

Faulhammer, Frank ; Konrad, Gerlinde ; Brankatschk, Ben ; Tahirovic, Sabina ; Knödler, Andreas ; Mayinger, Peter. / Cell growth-dependent coordination of lipid signaling and glycosylation is mediated by interactions between Sac1p and Dpm1p. In: Journal of Cell Biology. 2005 ; Vol. 168, No. 2. pp. 185-191.
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