Cell fusion-induced activation of interferon-stimulated genes is not required for restriction of a herpes simplex virus VP16/ICP0 mutant in heterokarya formed between permissive and restrictive cells

Meaghan Hancock, Karen L. Mossman, James R. Smiley

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction.

Original languageEnglish (US)
Pages (from-to)8976-8979
Number of pages4
JournalJournal of Virology
Volume83
Issue number17
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

herpes simplex
cell fusion
Cell Fusion
interferons
Simplexvirus
Interferons
viruses
mutants
Genes
Antiviral Agents
genes
cells
Phenotype
Membrane Fusion
Vero Cells
Osteosarcoma
phenotype
osteosarcoma
gene induction
Fibroblasts

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

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abstract = "Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction.",
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AU - Mossman, Karen L.

AU - Smiley, James R.

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AB - Herpes simplex virus VP16 and ICP0 mutants replicate efficiently in U2OS osteosarcoma cells but are restricted in other cell types. We previously showed that the restrictive phenotype is dominant in a transient cell fusion assay, suggesting that U2OS cells lack an antiviral mechanism present in other cells. Recent data indicate that unscheduled membrane fusion events can activate the expression of interferon-stimulated genes (ISGs) in fibroblasts, raising the possibility that our earlier results were due to a fusion-induced antiviral state. However, we show here that the permissive phenotype is also extinguished following fusion with Vero cells in the absence of ISG induction.

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