Cell-cycle characteristics of undifferentiated and differentiating embryonal carcinoma cells

Maurice J. Rosenstraus, Cynthia L. Sundell, R. Michael Liskay

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The cell-cycle parameters of undifferentiated and differentiating embryonal carcinoma cells were determined. Undifferentiated cultures of an F9 subclone, OTF9-63, had a 12-hr generation time, 10-hr S, and 2-hr G2 + M. G1 was less than 0.5 hr. In contrast, OTF9-63 cells induced to differentiate by treatment with retinoic acid had a 16.8-hr generation time, 12.5-hr S, 2-hr G2 + M, and 2.3-hr G1. Similar results were obtained with undifferentiated cultures and aggregation-induced differentiating cultures of PSA-1 cells. These data demonstrate that the undifferentiated stem cells have little or no G1, and that both G1 and S lengthen during differentiation.

Original languageEnglish (US)
Pages (from-to)516-520
Number of pages5
JournalDevelopmental Biology
Volume89
Issue number2
DOIs
StatePublished - Feb 1982

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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