Cell cycle analysis using numerical simulation of bivariate DNA/bromodeoxyuridine distributions

M. Yanagisawa, F. Dolbeare, T. Todoroki, Joe Gray

Research output: Chapter in Book/Report/Conference proceedingChapter

37 Citations (Scopus)

Abstract

This report describes a mathematical model of cell proliferation for simulation of bivariate DNA/bromodeoxyuridine (BrdUrd) distributions. The model formulates the change with time in the frequency of cells with any DNA content and in the amount of incorporated BrdUrd, according to given cytokinetic parameters, i.e., durations and dispersions of cell cycle phases and DNA synthesis rate during S-phase. We have applied this model to sequential DNA/BrdUrd distributions measured for Chinese hamster ovary cells asynchronously grown in vitro, 1) for 30 min in 10 μM BrdUrd followed by growth in BrdUrd-free medium for 0 to 24 h, or 2) during continuous incubation in 3 μM BrdUrd plus 30 μM thymidine for 2 to 24 h. The matches between the experimental and simulated distributions give the G1, S, G2M, and total cell cycle durations (and coefficients of variation) of 5.6 h (0.08), 7.0 h (0.07), 1.4 h (0.16), and 14.0 h (0.05), respectively. The model is shown to be useful for quantitative interpretation of the bivariate distributions.

Original languageEnglish (US)
Title of host publicationCytometry
Pages550-562
Number of pages13
Volume6
Edition6
StatePublished - 1985
Externally publishedYes

Fingerprint

Bromodeoxyuridine
Cell Cycle
DNA
Cricetulus
S Phase
Thymidine
Ovary
Theoretical Models
Cell Proliferation
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Yanagisawa, M., Dolbeare, F., Todoroki, T., & Gray, J. (1985). Cell cycle analysis using numerical simulation of bivariate DNA/bromodeoxyuridine distributions. In Cytometry (6 ed., Vol. 6, pp. 550-562)

Cell cycle analysis using numerical simulation of bivariate DNA/bromodeoxyuridine distributions. / Yanagisawa, M.; Dolbeare, F.; Todoroki, T.; Gray, Joe.

Cytometry. Vol. 6 6. ed. 1985. p. 550-562.

Research output: Chapter in Book/Report/Conference proceedingChapter

Yanagisawa, M, Dolbeare, F, Todoroki, T & Gray, J 1985, Cell cycle analysis using numerical simulation of bivariate DNA/bromodeoxyuridine distributions. in Cytometry. 6 edn, vol. 6, pp. 550-562.
Yanagisawa M, Dolbeare F, Todoroki T, Gray J. Cell cycle analysis using numerical simulation of bivariate DNA/bromodeoxyuridine distributions. In Cytometry. 6 ed. Vol. 6. 1985. p. 550-562
Yanagisawa, M. ; Dolbeare, F. ; Todoroki, T. ; Gray, Joe. / Cell cycle analysis using numerical simulation of bivariate DNA/bromodeoxyuridine distributions. Cytometry. Vol. 6 6. ed. 1985. pp. 550-562
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