Cell cycle analysis using flow cytometry

Joe Gray, F. Dolbeare, M. G. Pallavicini, W. Beisker, F. Waldman

Research output: Contribution to journalArticle

46 Scopus citations


This manuscript reviews the utility of flow cytometry for the study of cell proliferation. The applications of univariate DNA distribution analysis to cytokinetic studies of asynchronous and perturbed cell populations are discussed briefly. The newly developed technique for simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated bromodeoxyuridine is discussed in more detail. The cytochemistry required for this analysis is reviewed as are its applications to: (a) determination of the fractions of cells in the G1-, S- and G2 + M phases of the cell cycle; (b) determination of the G1-, S- and G2 + M phase durations and dispersions and growth fraction for asynchronous cells; (c) detection of ara-C resistant cells present at low frequency in an otherwise sensitive population; and (d) analysis of the cytokinetic response of a solid murine tumour to treatment in vivo with a cell cycle specific agent.

Original languageEnglish (US)
Pages (from-to)237-255
Number of pages19
JournalInternational Journal of Radiation Biology
Issue number2
Publication statusPublished - 1986
Externally publishedYes


ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Gray, J., Dolbeare, F., Pallavicini, M. G., Beisker, W., & Waldman, F. (1986). Cell cycle analysis using flow cytometry. International Journal of Radiation Biology, 49(2), 237-255. https://doi.org/10.1080/09553008514552531