Cell adhesion and integrin binding to recombinant human fibrillin-1

Martin Pfaff, Dieter P. Reinhardt, Lynn Sakai, Rupert Timpl

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Fibrillin-1 is a major constituent of tissue microfibrils that occur in most connective tissues, either in close association with or independent of elastin. To test possible cell-adhesive functions of this protein, we used recombinant human fibrillin-1 polypeptides produced in a mammalian expression system in cell attachment and solid-phase integrin binding assays. Fibrillin-1 polypeptides containing the single RGD sequence located in the fourth 8-cysteine domain, mediated distinct cell. adhesion of a variety of cell lines and bound to purified integrin αVβ3. Integrins αIIbβ3, α5β1, α2β1 and α1β1 did not interact with any of the recombinant fibrillin-1 peptides. Our results indicate a novel role for fibrillin-1 in cellular interactions mediated via an RGD motif that is appropriately exposed for recognition by integrin aVP3.

Original languageEnglish (US)
Pages (from-to)247-250
Number of pages4
JournalFEBS Letters
Volume384
Issue number3
DOIs
StatePublished - Apr 22 1996
Externally publishedYes

Fingerprint

Cell adhesion
Cell Adhesion
Integrins
Peptides
Tissue
Elastin
Microfibrils
Recombinant Proteins
Cysteine
Assays
Adhesives
Adhesion
Connective Tissue
Cells
Association reactions
Fibrillin-1
Cell Line

Keywords

  • Cell adhesion
  • Fibrillin-1
  • Integrin
  • RGD

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cell adhesion and integrin binding to recombinant human fibrillin-1. / Pfaff, Martin; Reinhardt, Dieter P.; Sakai, Lynn; Timpl, Rupert.

In: FEBS Letters, Vol. 384, No. 3, 22.04.1996, p. 247-250.

Research output: Contribution to journalArticle

Pfaff, Martin ; Reinhardt, Dieter P. ; Sakai, Lynn ; Timpl, Rupert. / Cell adhesion and integrin binding to recombinant human fibrillin-1. In: FEBS Letters. 1996 ; Vol. 384, No. 3. pp. 247-250.
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