TY - JOUR
T1 - CDX2 in the formation of the trophectoderm lineage in primate embryos
AU - Sritanaudomchai, Hathaitip
AU - Sparman, Michelle
AU - Tachibana, Masahito
AU - Clepper, Lisa
AU - Woodward, Joy
AU - Gokhale, Sumita
AU - Wolf, Don
AU - Hennebold, Jon
AU - Hurlbut, William
AU - Grompe, Markus
AU - Mitalipov, Shoukhrat
N1 - Funding Information:
The authors would like to acknowledge the Assisted Reproductive Technologies and Embryonic Stem Cell Core, Division of Animal Resources, Surgery Team, Endocrine Core, Imaging and Morphology Core and Molecular and Cellular Biology Core at the Oregon National Primate Research Center for providing expertise and services that contributed to this project. Funding was provided by start-up funds from Oregon National Primate Research Center, Oregon Stem Cell Center and National Institutes of Health grants RR00163, HD18185, HD057121-01A2, HD047721, HD047675 and HD058294.
PY - 2009/11/1
Y1 - 2009/11/1
N2 - The first lineage decision during mammalian development is the establishment of the trophectoderm (TE) and the inner cell mass (ICM). The caudal-type homeodomain protein Cdx2 is implicated in the formation and maintenance of the TE in the mouse. However, the role of CDX2 during early embryonic development in primates is unknown. Here, we demonstrated that CDX2 mRNA levels were detectable in rhesus monkey oocytes, significantly upregulated in pronuclear stage zygotes, diminished in early cleaving embryos but restored again in compact morula and blastocyst stages. CDX2 protein was localized to the nucleus of TE cells but absent altogether in the ICM. Knockdown of CDX2 in monkey oocytes resulted in formation of early blastocyst-like embryos that failed to expand and ceased development. However, the ICM lineage of CDX2-deficient embryos supported the isolation of functional embryonic stem cells. These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development.
AB - The first lineage decision during mammalian development is the establishment of the trophectoderm (TE) and the inner cell mass (ICM). The caudal-type homeodomain protein Cdx2 is implicated in the formation and maintenance of the TE in the mouse. However, the role of CDX2 during early embryonic development in primates is unknown. Here, we demonstrated that CDX2 mRNA levels were detectable in rhesus monkey oocytes, significantly upregulated in pronuclear stage zygotes, diminished in early cleaving embryos but restored again in compact morula and blastocyst stages. CDX2 protein was localized to the nucleus of TE cells but absent altogether in the ICM. Knockdown of CDX2 in monkey oocytes resulted in formation of early blastocyst-like embryos that failed to expand and ceased development. However, the ICM lineage of CDX2-deficient embryos supported the isolation of functional embryonic stem cells. These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development.
KW - CDX2
KW - Embryo
KW - Embryonic stem cells
KW - Primates
KW - Trophectoderm
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U2 - 10.1016/j.ydbio.2009.08.025
DO - 10.1016/j.ydbio.2009.08.025
M3 - Article
C2 - 19733166
AN - SCOPUS:70349782291
SN - 0012-1606
VL - 335
SP - 179
EP - 187
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -