CDX2 in the formation of the trophectoderm lineage in primate embryos

Hathaitip Sritanaudomchai, Michelle Sparman, Masahito Tachibana, Lisa Clepper, Joy Woodward, Sumita Gokhale, Don Wolf, Jon Hennebold, William Hurlbut, Markus Grompe, Shoukhrat Mitalipov

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The first lineage decision during mammalian development is the establishment of the trophectoderm (TE) and the inner cell mass (ICM). The caudal-type homeodomain protein Cdx2 is implicated in the formation and maintenance of the TE in the mouse. However, the role of CDX2 during early embryonic development in primates is unknown. Here, we demonstrated that CDX2 mRNA levels were detectable in rhesus monkey oocytes, significantly upregulated in pronuclear stage zygotes, diminished in early cleaving embryos but restored again in compact morula and blastocyst stages. CDX2 protein was localized to the nucleus of TE cells but absent altogether in the ICM. Knockdown of CDX2 in monkey oocytes resulted in formation of early blastocyst-like embryos that failed to expand and ceased development. However, the ICM lineage of CDX2-deficient embryos supported the isolation of functional embryonic stem cells. These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalDevelopmental Biology
Volume335
Issue number1
DOIs
StatePublished - Nov 1 2009

Fingerprint

Primates
Embryonic Structures
Blastocyst
Oocytes
Embryonic Development
Homeodomain Proteins
Morula
Zygote
Cell Lineage
Embryonic Stem Cells
Macaca mulatta
Cell Nucleus
Haplorhini
Maintenance
Messenger RNA
CDX2 Transcription Factor

Keywords

  • CDX2
  • Embryo
  • Embryonic stem cells
  • Primates
  • Trophectoderm

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Sritanaudomchai, H., Sparman, M., Tachibana, M., Clepper, L., Woodward, J., Gokhale, S., ... Mitalipov, S. (2009). CDX2 in the formation of the trophectoderm lineage in primate embryos. Developmental Biology, 335(1), 179-187. https://doi.org/10.1016/j.ydbio.2009.08.025

CDX2 in the formation of the trophectoderm lineage in primate embryos. / Sritanaudomchai, Hathaitip; Sparman, Michelle; Tachibana, Masahito; Clepper, Lisa; Woodward, Joy; Gokhale, Sumita; Wolf, Don; Hennebold, Jon; Hurlbut, William; Grompe, Markus; Mitalipov, Shoukhrat.

In: Developmental Biology, Vol. 335, No. 1, 01.11.2009, p. 179-187.

Research output: Contribution to journalArticle

Sritanaudomchai, H, Sparman, M, Tachibana, M, Clepper, L, Woodward, J, Gokhale, S, Wolf, D, Hennebold, J, Hurlbut, W, Grompe, M & Mitalipov, S 2009, 'CDX2 in the formation of the trophectoderm lineage in primate embryos', Developmental Biology, vol. 335, no. 1, pp. 179-187. https://doi.org/10.1016/j.ydbio.2009.08.025
Sritanaudomchai H, Sparman M, Tachibana M, Clepper L, Woodward J, Gokhale S et al. CDX2 in the formation of the trophectoderm lineage in primate embryos. Developmental Biology. 2009 Nov 1;335(1):179-187. https://doi.org/10.1016/j.ydbio.2009.08.025
Sritanaudomchai, Hathaitip ; Sparman, Michelle ; Tachibana, Masahito ; Clepper, Lisa ; Woodward, Joy ; Gokhale, Sumita ; Wolf, Don ; Hennebold, Jon ; Hurlbut, William ; Grompe, Markus ; Mitalipov, Shoukhrat. / CDX2 in the formation of the trophectoderm lineage in primate embryos. In: Developmental Biology. 2009 ; Vol. 335, No. 1. pp. 179-187.
@article{6cc4185ab9e048ca80f294a881169f07,
title = "CDX2 in the formation of the trophectoderm lineage in primate embryos",
abstract = "The first lineage decision during mammalian development is the establishment of the trophectoderm (TE) and the inner cell mass (ICM). The caudal-type homeodomain protein Cdx2 is implicated in the formation and maintenance of the TE in the mouse. However, the role of CDX2 during early embryonic development in primates is unknown. Here, we demonstrated that CDX2 mRNA levels were detectable in rhesus monkey oocytes, significantly upregulated in pronuclear stage zygotes, diminished in early cleaving embryos but restored again in compact morula and blastocyst stages. CDX2 protein was localized to the nucleus of TE cells but absent altogether in the ICM. Knockdown of CDX2 in monkey oocytes resulted in formation of early blastocyst-like embryos that failed to expand and ceased development. However, the ICM lineage of CDX2-deficient embryos supported the isolation of functional embryonic stem cells. These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development.",
keywords = "CDX2, Embryo, Embryonic stem cells, Primates, Trophectoderm",
author = "Hathaitip Sritanaudomchai and Michelle Sparman and Masahito Tachibana and Lisa Clepper and Joy Woodward and Sumita Gokhale and Don Wolf and Jon Hennebold and William Hurlbut and Markus Grompe and Shoukhrat Mitalipov",
year = "2009",
month = "11",
day = "1",
doi = "10.1016/j.ydbio.2009.08.025",
language = "English (US)",
volume = "335",
pages = "179--187",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - CDX2 in the formation of the trophectoderm lineage in primate embryos

AU - Sritanaudomchai, Hathaitip

AU - Sparman, Michelle

AU - Tachibana, Masahito

AU - Clepper, Lisa

AU - Woodward, Joy

AU - Gokhale, Sumita

AU - Wolf, Don

AU - Hennebold, Jon

AU - Hurlbut, William

AU - Grompe, Markus

AU - Mitalipov, Shoukhrat

PY - 2009/11/1

Y1 - 2009/11/1

N2 - The first lineage decision during mammalian development is the establishment of the trophectoderm (TE) and the inner cell mass (ICM). The caudal-type homeodomain protein Cdx2 is implicated in the formation and maintenance of the TE in the mouse. However, the role of CDX2 during early embryonic development in primates is unknown. Here, we demonstrated that CDX2 mRNA levels were detectable in rhesus monkey oocytes, significantly upregulated in pronuclear stage zygotes, diminished in early cleaving embryos but restored again in compact morula and blastocyst stages. CDX2 protein was localized to the nucleus of TE cells but absent altogether in the ICM. Knockdown of CDX2 in monkey oocytes resulted in formation of early blastocyst-like embryos that failed to expand and ceased development. However, the ICM lineage of CDX2-deficient embryos supported the isolation of functional embryonic stem cells. These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development.

AB - The first lineage decision during mammalian development is the establishment of the trophectoderm (TE) and the inner cell mass (ICM). The caudal-type homeodomain protein Cdx2 is implicated in the formation and maintenance of the TE in the mouse. However, the role of CDX2 during early embryonic development in primates is unknown. Here, we demonstrated that CDX2 mRNA levels were detectable in rhesus monkey oocytes, significantly upregulated in pronuclear stage zygotes, diminished in early cleaving embryos but restored again in compact morula and blastocyst stages. CDX2 protein was localized to the nucleus of TE cells but absent altogether in the ICM. Knockdown of CDX2 in monkey oocytes resulted in formation of early blastocyst-like embryos that failed to expand and ceased development. However, the ICM lineage of CDX2-deficient embryos supported the isolation of functional embryonic stem cells. These results provide evidence that CDX2 plays an essential role in functional TE formation during primate embryonic development.

KW - CDX2

KW - Embryo

KW - Embryonic stem cells

KW - Primates

KW - Trophectoderm

UR - http://www.scopus.com/inward/record.url?scp=70349782291&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349782291&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2009.08.025

DO - 10.1016/j.ydbio.2009.08.025

M3 - Article

C2 - 19733166

AN - SCOPUS:70349782291

VL - 335

SP - 179

EP - 187

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -