CD45 alternative exon expression in murine and human CD4+ T cell subsets

Paul R. Rogers, Suzie Pilapil, Kyoko Hayakawa, Paul L. Romain, David Parker

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Leukocytes express a family of high m.w. glycoproteins called leukocyte common Ag (CD45), which are involved in phosphotyrosine signal transduction. Antibodies to different CD45 isoforms distinguish functionally different CD4+ T cell subsets in humans, rats, and mice. Selected protein isoforms are expressed through a process of exon splicing that is cell-type and differentiation-state specific. Splicing of the three variable exons, A, B, and C, which encode amino acids located near the extracellular amino terminus of the protein, potentially results in generation of eight different mRNA transcripts. The purpose of the present study was to determine the relative levels of all eight different CD45 transcripts present in a panel of murine CD4+ T cell lines and normal murine and human CD4+ T cell subsets separated with antibodies to CD45 variable exons. We show, as expected, that the broad features of CD45 surface isoform expression in these cells can be accounted for by the relative amounts of the eight differentially spliced transcripts. Unexpectedly, all the differences in CD45 isoform expression among the CD4+ T cell subpopulations that we measured could be accounted for by differences in the overall level of variable exon expression. We did not see differences among T cell populations in the relative expression of particular variable exons. Exon B was always found in greater abundance than exons C or A. Of the dual exon species, only AB and BC were found in CD4+ T cells. The AC species was undetectable. Human CD4+ T cells, especially those in the naive subset, express higher levels of CD45 variable exons than murine CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)4054-4065
Number of pages12
JournalJournal of Immunology
Volume148
Issue number12
StatePublished - Jun 15 1992
Externally publishedYes

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T-Lymphocyte Subsets
Exons
T-Lymphocytes
Protein Isoforms
Leukocytes
Phosphotyrosine
Antibodies
Cell Differentiation
Signal Transduction
Glycoproteins
Amino Acids
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Rogers, P. R., Pilapil, S., Hayakawa, K., Romain, P. L., & Parker, D. (1992). CD45 alternative exon expression in murine and human CD4+ T cell subsets. Journal of Immunology, 148(12), 4054-4065.

CD45 alternative exon expression in murine and human CD4+ T cell subsets. / Rogers, Paul R.; Pilapil, Suzie; Hayakawa, Kyoko; Romain, Paul L.; Parker, David.

In: Journal of Immunology, Vol. 148, No. 12, 15.06.1992, p. 4054-4065.

Research output: Contribution to journalArticle

Rogers, PR, Pilapil, S, Hayakawa, K, Romain, PL & Parker, D 1992, 'CD45 alternative exon expression in murine and human CD4+ T cell subsets', Journal of Immunology, vol. 148, no. 12, pp. 4054-4065.
Rogers PR, Pilapil S, Hayakawa K, Romain PL, Parker D. CD45 alternative exon expression in murine and human CD4+ T cell subsets. Journal of Immunology. 1992 Jun 15;148(12):4054-4065.
Rogers, Paul R. ; Pilapil, Suzie ; Hayakawa, Kyoko ; Romain, Paul L. ; Parker, David. / CD45 alternative exon expression in murine and human CD4+ T cell subsets. In: Journal of Immunology. 1992 ; Vol. 148, No. 12. pp. 4054-4065.
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