CD40L is transferred to antigen-presenting B cells during delivery of T-cell help

Jennifer L. Gardell, David Parker

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The delivery of T-cell help to B cells is antigen-specific, MHC-restricted, and CD40L (CD154) dependent. It has been thought that when a T cell recognizes an antigen-presenting B cell, CD40L expressed on the T-cell surface engages with CD40 on the surface of B cells as long as the cells remain conjugated. By adding fluorescently labeled anti-CD40L antibody during overnight incubation of antigen-presenting B cells with antigen-specific T cells, we discovered that CD40L does not remain on the surface of the T cell, but it is transferred to and endocytosed by B cells receiving T-cell help. In the presence of anti-CD40L antibody, transferred CD40L is nearly absent on bystander B cells that are not presenting antigen, and the bystander cells do not become activated. Because transfer of CD40L to B cells correlates with B-cell activation, we speculate that persistence of helper T-cell-derived CD40L on or in B cells could permit sustained CD40 signaling enabling survival and proliferation of antigen-presenting B cells following brief interactions with helper T cells in vivo in germinal centers.

Original languageEnglish (US)
Pages (from-to)41-50
Number of pages10
JournalEuropean Journal of Immunology
Volume47
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

CD40 Ligand
Antigen-Presenting Cells
B-Lymphocytes
T-Lymphocytes
Helper-Inducer T-Lymphocytes
Anti-Idiotypic Antibodies
Antigens
Germinal Center
Endocytosis

Keywords

  • CD154
  • CD40 ligand
  • CD40L
  • Immunological synapse
  • Membrane transfer
  • T-cell help

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

CD40L is transferred to antigen-presenting B cells during delivery of T-cell help. / Gardell, Jennifer L.; Parker, David.

In: European Journal of Immunology, Vol. 47, No. 1, 01.01.2017, p. 41-50.

Research output: Contribution to journalArticle

Gardell, Jennifer L. ; Parker, David. / CD40L is transferred to antigen-presenting B cells during delivery of T-cell help. In: European Journal of Immunology. 2017 ; Vol. 47, No. 1. pp. 41-50.
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