CD40 ligand inhibits Fas/CD95-mediated apoptosis of human blood-derived dendritic cells

Thelma A. Koppi, Teresa Tough-Bement, David Lewinsohn, David H. Lynch, Mark R. Alderson

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Dendritic cells (DC) are considered to be the mast potent antigen-presenting cells (APC) in the immune system. In this study, we analyzed the regulation of apoptosis of human peripheral blood-derived DC. DC were generated from adherent peripheral blood mononuclear cells that had been cultured for 7 days with granulocyte-macrophage colony-stimulating factor and interleukin-4. These cells displayed phenotypic properties of DC, including dendritic processes, expression of CD1a and lack of expression of CD14, and were very potent at presenting soluble antigens to T cells. Blood-derived DC were demonstrated to express the Fas/CD95 antigen and an agonist antibody to CD95 strongly induced apoptotic cell death in these cells. Soluble trimeric CD40 ligand potently inhibited both CD95-mediated and spontaneous apoptosis in DC. The data suggest that interactions between members of the tumor necrosis factor family of ligands expressed by T cells with their receptors on DC play an important role in the regulation of apoptosis in DC during antigen presentation and may, therefore, regulate the duration of T cell expansion and cytokine production.

Original languageEnglish (US)
Pages (from-to)3161-3165
Number of pages5
JournalEuropean Journal of Immunology
Volume27
Issue number12
DOIs
StatePublished - Dec 1997
Externally publishedYes

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CD40 Ligand
Dendritic Cells
Apoptosis
CD95 Antigens
T-Lymphocytes
Antigen Presentation
Antigen-Presenting Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Immune System
Blood Cells
Cell Death
Tumor Necrosis Factor-alpha
Cytokines
Ligands
Antigens
Antibodies

Keywords

  • Cytometry
  • Granulocyte-macrophage colony-stimulating factor
  • Hoechst 33342
  • Propidium iodide

ASJC Scopus subject areas

  • Immunology

Cite this

CD40 ligand inhibits Fas/CD95-mediated apoptosis of human blood-derived dendritic cells. / Koppi, Thelma A.; Tough-Bement, Teresa; Lewinsohn, David; Lynch, David H.; Alderson, Mark R.

In: European Journal of Immunology, Vol. 27, No. 12, 12.1997, p. 3161-3165.

Research output: Contribution to journalArticle

Koppi, Thelma A. ; Tough-Bement, Teresa ; Lewinsohn, David ; Lynch, David H. ; Alderson, Mark R. / CD40 ligand inhibits Fas/CD95-mediated apoptosis of human blood-derived dendritic cells. In: European Journal of Immunology. 1997 ; Vol. 27, No. 12. pp. 3161-3165.
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AU - Alderson, Mark R.

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