CD40 ligand inhibits Fas/CD95-mediated apoptosis of human blood-derived dendritic cells

Thelma A. Koppi, Teresa Tough-Bement, David M. Lewinsohn, David H. Lynch, Mark R. Alderson

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

Dendritic cells (DC) are considered to be the mast potent antigen-presenting cells (APC) in the immune system. In this study, we analyzed the regulation of apoptosis of human peripheral blood-derived DC. DC were generated from adherent peripheral blood mononuclear cells that had been cultured for 7 days with granulocyte-macrophage colony-stimulating factor and interleukin-4. These cells displayed phenotypic properties of DC, including dendritic processes, expression of CD1a and lack of expression of CD14, and were very potent at presenting soluble antigens to T cells. Blood-derived DC were demonstrated to express the Fas/CD95 antigen and an agonist antibody to CD95 strongly induced apoptotic cell death in these cells. Soluble trimeric CD40 ligand potently inhibited both CD95-mediated and spontaneous apoptosis in DC. The data suggest that interactions between members of the tumor necrosis factor family of ligands expressed by T cells with their receptors on DC play an important role in the regulation of apoptosis in DC during antigen presentation and may, therefore, regulate the duration of T cell expansion and cytokine production.

Original languageEnglish (US)
Pages (from-to)3161-3165
Number of pages5
JournalEuropean Journal of Immunology
Volume27
Issue number12
DOIs
StatePublished - Dec 1997

Keywords

  • Cytometry
  • Granulocyte-macrophage colony-stimulating factor
  • Hoechst 33342
  • Propidium iodide

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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