CD40 ligand-deficient mice generate a normal primary cytotoxic T- lymphocyte response but a defective humoral response to a viral infection

Jason K. Whitmire, Mark Slifka, Iqbal S. Grewal, Richard A. Flavell, Rafi Ahmed

Research output: Contribution to journalArticle

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Abstract

CD40 ligand is expressed on activated T cells and interacts with CD40 on B cells and monocytes. It is not known what role CD40 ligand plays in the generation of immune responses to viral infection. To address this issue, we examined virus-specific T- and B-cell responses in CD40 ligand-deficient (CD40L-/-) mice following infection with lymphocytic choriomeningitis virus (LCMV). We found that primary anti-LCMV specific antibody responses were severely impaired in CD40L-/- mice, with the defect being most striking for antibody of the immunoglobulin G1 (IgG1) isotype. Interestingly, low levels of LCMV-specific antibodies of the IgG2a, IgG2b, and IgG3 isotypes were made in the CD40L-/- mice, showing that IgG1 responses are totally dependent on CD40L but that at least some IgG2a, IgG2b, and IgG3 responses can be CD40L independent. However, unlike CD40L+/+ mice, CD40L-/- mice were unable to sustain virus-specific antibody responses and showed a gradual decline in serum antibody levels over time. The CD40L-/- mice were also deficient in the generation of memory B cells. In contrast to the severely impaired humoral responses, CD40L-/- mice generated potent virus-specific CD8+ cytotoxic T- lymphocyte responses after LCMV infection and were able to clear the virus. These results show that CD40L does not play a rule in generating primary virus-specific CD8+ cytotoxic T-lymphocyte responses but does affect the primary antibody response and the generation of memory B cells.

Original languageEnglish (US)
Pages (from-to)8375-8381
Number of pages7
JournalJournal of Virology
Volume70
Issue number12
StatePublished - Dec 1996
Externally publishedYes

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CD40 Ligand
cytotoxic T-lymphocytes
Cytotoxic T-Lymphocytes
Virus Diseases
humoral immunity
Lymphocytic choriomeningitis virus
mice
infection
antibodies
B-lymphocytes
viruses
Viruses
B-Lymphocytes
immunoglobulins
Antibody Formation
T-lymphocytes
ligands
Antibodies
Immunoglobulin G
monocytes

ASJC Scopus subject areas

  • Immunology

Cite this

CD40 ligand-deficient mice generate a normal primary cytotoxic T- lymphocyte response but a defective humoral response to a viral infection. / Whitmire, Jason K.; Slifka, Mark; Grewal, Iqbal S.; Flavell, Richard A.; Ahmed, Rafi.

In: Journal of Virology, Vol. 70, No. 12, 12.1996, p. 8375-8381.

Research output: Contribution to journalArticle

Whitmire, Jason K. ; Slifka, Mark ; Grewal, Iqbal S. ; Flavell, Richard A. ; Ahmed, Rafi. / CD40 ligand-deficient mice generate a normal primary cytotoxic T- lymphocyte response but a defective humoral response to a viral infection. In: Journal of Virology. 1996 ; Vol. 70, No. 12. pp. 8375-8381.
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