CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis

François Marchildon, Neena Lala, Grace Li, Catherine St.-Louis, Daniel Lamothe, Charles Keller, Nadine Wiper-Bergeron

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Upon injury, muscle satellite cells become activated and produce skeletal muscle precursors that engage in myogenesis. We demonstrate that the transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in the satellite cells of healthy muscle. C/EBPβ expression is regulated during myogenesis such that C/EBPβ is rapidly and massively downregulated upon induction to differentiate. Furthermore, persistent expression of C/EBPβ in myoblasts potently inhibits differentiation at least in part through the inhibition of MyoD protein function and stability. As a consequence, myogenic factor expression, myosin heavy chain expression, and fusogenic activity were reduced in C/EBPβ-overexpressing cells. Using knockout models, we demonstrate that loss of Cebpb expression in satellite cells results in precocious differentiation of myoblasts in growth conditions and greater cell fusion upon differentiation. In vivo, loss of Cebpb expression in satellite cells resulted in larger muscle fiber cross-sectional area and improved repair after muscle injury. Our results support the notion that C/EBPβ inhibits myogenic differentiation and that its levels must be reduced to allow for activation of MyoD target genes and the progression of differentiation.

Original languageEnglish (US)
Pages (from-to)2619-2630
Number of pages12
JournalStem Cells
Volume30
Issue number12
DOIs
StatePublished - Dec 2012

Fingerprint

CCAAT-Enhancer-Binding Protein-beta
Muscle Development
Myoblasts
Muscle Cells
MyoD Protein
Muscles
Myosin Heavy Chains
Cell Fusion
Protein Stability
Wounds and Injuries
Skeletal Muscle
Transcription Factors
Down-Regulation
Growth
Genes

Keywords

  • CCAAT-enhancer binding protein beta
  • Muscle development
  • MyoD1 myogenic differentiation protein
  • Satellite cells
  • Skeletal muscle

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Marchildon, F., Lala, N., Li, G., St.-Louis, C., Lamothe, D., Keller, C., & Wiper-Bergeron, N. (2012). CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis. Stem Cells, 30(12), 2619-2630. https://doi.org/10.1002/stem.1248

CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis. / Marchildon, François; Lala, Neena; Li, Grace; St.-Louis, Catherine; Lamothe, Daniel; Keller, Charles; Wiper-Bergeron, Nadine.

In: Stem Cells, Vol. 30, No. 12, 12.2012, p. 2619-2630.

Research output: Contribution to journalArticle

Marchildon, F, Lala, N, Li, G, St.-Louis, C, Lamothe, D, Keller, C & Wiper-Bergeron, N 2012, 'CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis', Stem Cells, vol. 30, no. 12, pp. 2619-2630. https://doi.org/10.1002/stem.1248
Marchildon F, Lala N, Li G, St.-Louis C, Lamothe D, Keller C et al. CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis. Stem Cells. 2012 Dec;30(12):2619-2630. https://doi.org/10.1002/stem.1248
Marchildon, François ; Lala, Neena ; Li, Grace ; St.-Louis, Catherine ; Lamothe, Daniel ; Keller, Charles ; Wiper-Bergeron, Nadine. / CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis. In: Stem Cells. 2012 ; Vol. 30, No. 12. pp. 2619-2630.
@article{1eab8bfeea81415c838e2079a90bcdfa,
title = "CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis",
abstract = "Upon injury, muscle satellite cells become activated and produce skeletal muscle precursors that engage in myogenesis. We demonstrate that the transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in the satellite cells of healthy muscle. C/EBPβ expression is regulated during myogenesis such that C/EBPβ is rapidly and massively downregulated upon induction to differentiate. Furthermore, persistent expression of C/EBPβ in myoblasts potently inhibits differentiation at least in part through the inhibition of MyoD protein function and stability. As a consequence, myogenic factor expression, myosin heavy chain expression, and fusogenic activity were reduced in C/EBPβ-overexpressing cells. Using knockout models, we demonstrate that loss of Cebpb expression in satellite cells results in precocious differentiation of myoblasts in growth conditions and greater cell fusion upon differentiation. In vivo, loss of Cebpb expression in satellite cells resulted in larger muscle fiber cross-sectional area and improved repair after muscle injury. Our results support the notion that C/EBPβ inhibits myogenic differentiation and that its levels must be reduced to allow for activation of MyoD target genes and the progression of differentiation.",
keywords = "CCAAT-enhancer binding protein beta, Muscle development, MyoD1 myogenic differentiation protein, Satellite cells, Skeletal muscle",
author = "Fran{\cc}ois Marchildon and Neena Lala and Grace Li and Catherine St.-Louis and Daniel Lamothe and Charles Keller and Nadine Wiper-Bergeron",
year = "2012",
month = "12",
doi = "10.1002/stem.1248",
language = "English (US)",
volume = "30",
pages = "2619--2630",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "12",

}

TY - JOUR

T1 - CCAAT/enhancer binding protein beta is expressed in satellite cells and controls myogenesis

AU - Marchildon, François

AU - Lala, Neena

AU - Li, Grace

AU - St.-Louis, Catherine

AU - Lamothe, Daniel

AU - Keller, Charles

AU - Wiper-Bergeron, Nadine

PY - 2012/12

Y1 - 2012/12

N2 - Upon injury, muscle satellite cells become activated and produce skeletal muscle precursors that engage in myogenesis. We demonstrate that the transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in the satellite cells of healthy muscle. C/EBPβ expression is regulated during myogenesis such that C/EBPβ is rapidly and massively downregulated upon induction to differentiate. Furthermore, persistent expression of C/EBPβ in myoblasts potently inhibits differentiation at least in part through the inhibition of MyoD protein function and stability. As a consequence, myogenic factor expression, myosin heavy chain expression, and fusogenic activity were reduced in C/EBPβ-overexpressing cells. Using knockout models, we demonstrate that loss of Cebpb expression in satellite cells results in precocious differentiation of myoblasts in growth conditions and greater cell fusion upon differentiation. In vivo, loss of Cebpb expression in satellite cells resulted in larger muscle fiber cross-sectional area and improved repair after muscle injury. Our results support the notion that C/EBPβ inhibits myogenic differentiation and that its levels must be reduced to allow for activation of MyoD target genes and the progression of differentiation.

AB - Upon injury, muscle satellite cells become activated and produce skeletal muscle precursors that engage in myogenesis. We demonstrate that the transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in the satellite cells of healthy muscle. C/EBPβ expression is regulated during myogenesis such that C/EBPβ is rapidly and massively downregulated upon induction to differentiate. Furthermore, persistent expression of C/EBPβ in myoblasts potently inhibits differentiation at least in part through the inhibition of MyoD protein function and stability. As a consequence, myogenic factor expression, myosin heavy chain expression, and fusogenic activity were reduced in C/EBPβ-overexpressing cells. Using knockout models, we demonstrate that loss of Cebpb expression in satellite cells results in precocious differentiation of myoblasts in growth conditions and greater cell fusion upon differentiation. In vivo, loss of Cebpb expression in satellite cells resulted in larger muscle fiber cross-sectional area and improved repair after muscle injury. Our results support the notion that C/EBPβ inhibits myogenic differentiation and that its levels must be reduced to allow for activation of MyoD target genes and the progression of differentiation.

KW - CCAAT-enhancer binding protein beta

KW - Muscle development

KW - MyoD1 myogenic differentiation protein

KW - Satellite cells

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=84870335301&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870335301&partnerID=8YFLogxK

U2 - 10.1002/stem.1248

DO - 10.1002/stem.1248

M3 - Article

C2 - 23034923

AN - SCOPUS:84870335301

VL - 30

SP - 2619

EP - 2630

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 12

ER -