Cathepsin G Activity as a New Marker for Detecting Airway Inflammation by Microscopy and Flow Cytometry

Matteo Guerra, Dario Frey, Matthias Hagner, Susanne Dittrich, Michelle Paulsen, Marcus A. Mall, Carsten Schultz

Research output: Contribution to journalArticle

Abstract

Muco-obstructive lung diseases feature extensive bronchiectasis due to the uncontrolled release of neutrophil serine proteases into the airways. To assess if cathepsin G (CG) is a novel key player in chronic lung inflammation, we developed membrane-bound (mSAM) and soluble (sSAM) FRET reporters. The probes quantitatively revealed elevated CG activity in samples from 46 patients. For future basic science and personalized clinical applications, we developed a rapid, highly informative, and easily translatable small-molecule FRET flow cytometry assay for monitoring protease activity including cathepsin G. We demonstrated that mSAM distinguished healthy from patient cells by FRET-based flow cytometry with excellent correlation to confocal microscopy data.

Original languageEnglish (US)
JournalACS Central Science
DOIs
StatePublished - Jan 1 2019

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Cathepsin G
Flow cytometry
Microscopic examination
Pulmonary diseases
Confocal microscopy
Serine Proteases
Assays
Peptide Hydrolases
Membranes
Molecules
Monitoring
Cathepsins

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Cathepsin G Activity as a New Marker for Detecting Airway Inflammation by Microscopy and Flow Cytometry. / Guerra, Matteo; Frey, Dario; Hagner, Matthias; Dittrich, Susanne; Paulsen, Michelle; Mall, Marcus A.; Schultz, Carsten.

In: ACS Central Science, 01.01.2019.

Research output: Contribution to journalArticle

Guerra, Matteo ; Frey, Dario ; Hagner, Matthias ; Dittrich, Susanne ; Paulsen, Michelle ; Mall, Marcus A. ; Schultz, Carsten. / Cathepsin G Activity as a New Marker for Detecting Airway Inflammation by Microscopy and Flow Cytometry. In: ACS Central Science. 2019.
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