Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense

Shida Yousefi, Jeffrey (Jeff) Gold, Nicola Andina, James J. Lee, Ann M. Kelly, Evelyne Kozlowski, Inès Schmid, Alex Straumann, Janine Reichenbach, Gerald J. Gleich, Hans Uwe Simon

Research output: Contribution to journalArticle

468 Citations (Scopus)

Abstract

Although eosinophils are considered useful in defense mechanisms against parasites, their exact function in innate immunity remains unclear. The aim of this study is to better understand the role of eosinophils within the gastrointestinal immune system. We show here that lipopolysaccharide from Gram-negative bacteria activates interleukin-5 (IL-5)- or interferon-γ- primed eosinophils to release mitochondrial DNA in a reactive oxygen species-dependent manner, but independent of eosinophil death. Notably, the process of DNA release occurs rapidly in a catapult-like manner - in less than one second. In the extracellular space, the mitochondrial DNA and the granule proteins form extracellular structures able to bind and kill bacteria both in vitro and under inflammatory conditions in vivo. Moreover, after cecal ligation and puncture, Il5-transgenic but not wild-type mice show intestinal eosinophil infiltration and extracellular DNA deposition in association with protection against microbial sepsis. These data suggest a previously undescribed mechanism of eosinophil-mediated innate immune responses that might be crucial for maintaining the intestinal barrier function after inflammation-associated epithelial cell damage, preventing the host from uncontrolled invasion of bacteria.

Original languageEnglish (US)
Pages (from-to)949-953
Number of pages5
JournalNature Medicine
Volume14
Issue number9
DOIs
StatePublished - Sep 2008

Fingerprint

Mitochondrial DNA
Eosinophils
Bacteria
Immune system
DNA
Interleukin-5
Infiltration
Innate Immunity
Interferons
Lipopolysaccharides
Reactive Oxygen Species
Extracellular Space
Gram-Negative Bacteria
Punctures
Ligation
Immune System
Sepsis
Parasites
Proteins
Epithelial Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Yousefi, S., Gold, J. J., Andina, N., Lee, J. J., Kelly, A. M., Kozlowski, E., ... Simon, H. U. (2008). Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense. Nature Medicine, 14(9), 949-953. https://doi.org/10.1038/nm.1855

Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense. / Yousefi, Shida; Gold, Jeffrey (Jeff); Andina, Nicola; Lee, James J.; Kelly, Ann M.; Kozlowski, Evelyne; Schmid, Inès; Straumann, Alex; Reichenbach, Janine; Gleich, Gerald J.; Simon, Hans Uwe.

In: Nature Medicine, Vol. 14, No. 9, 09.2008, p. 949-953.

Research output: Contribution to journalArticle

Yousefi, S, Gold, JJ, Andina, N, Lee, JJ, Kelly, AM, Kozlowski, E, Schmid, I, Straumann, A, Reichenbach, J, Gleich, GJ & Simon, HU 2008, 'Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense', Nature Medicine, vol. 14, no. 9, pp. 949-953. https://doi.org/10.1038/nm.1855
Yousefi, Shida ; Gold, Jeffrey (Jeff) ; Andina, Nicola ; Lee, James J. ; Kelly, Ann M. ; Kozlowski, Evelyne ; Schmid, Inès ; Straumann, Alex ; Reichenbach, Janine ; Gleich, Gerald J. ; Simon, Hans Uwe. / Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense. In: Nature Medicine. 2008 ; Vol. 14, No. 9. pp. 949-953.
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