Catalytic domains of tyrosine kinases determine the phosphorylation sites within c-Cbl

A. H. Grossmann, K. S. Kolibaba, S. G. Willis, A. S. Corbin, W. S. Langdon, M. W N Deininger, Brian Druker

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Catalytic (SH1) domains of protein tyrosine kinases (PTKs) demonstrate specificity for peptide substrates. Whether SH1 domains differentiate between tyrosines in a physiological substrate has not been confirmed. Using purified proteins, we studied the ability of Syk, Fyn, and Abl to differentiate between tyrosines in a common PTK substrate, c-Cbl. We found that each kinase produced a distinct pattern of c-Cbl phosphorylation, which altered the phosphotyrosine-dependent interactions between c-Cbl and CrkL or phosphatidylinositol 3-kinase (PI3-K). Our data support the concept that SH1 domains determine the final sites of phosphorylation once PTKs reach their target proteins.

Original languageEnglish (US)
Pages (from-to)555-562
Number of pages8
JournalFEBS Letters
Volume577
Issue number3
DOIs
StatePublished - Nov 19 2004

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Phosphorylation
src Homology Domains
Protein-Tyrosine Kinases
Catalytic Domain
Tyrosine
Substrates
Phosphatidylinositol 3-Kinase
Phosphotyrosine
Substrate Specificity
Proteins
Phosphotransferases
Peptides

Keywords

  • c-Cbl phosphorylation
  • Protein tyrosine kinase
  • Substrate specificity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Grossmann, A. H., Kolibaba, K. S., Willis, S. G., Corbin, A. S., Langdon, W. S., Deininger, M. W. N., & Druker, B. (2004). Catalytic domains of tyrosine kinases determine the phosphorylation sites within c-Cbl. FEBS Letters, 577(3), 555-562. https://doi.org/10.1016/j.febslet.2004.10.054

Catalytic domains of tyrosine kinases determine the phosphorylation sites within c-Cbl. / Grossmann, A. H.; Kolibaba, K. S.; Willis, S. G.; Corbin, A. S.; Langdon, W. S.; Deininger, M. W N; Druker, Brian.

In: FEBS Letters, Vol. 577, No. 3, 19.11.2004, p. 555-562.

Research output: Contribution to journalArticle

Grossmann, AH, Kolibaba, KS, Willis, SG, Corbin, AS, Langdon, WS, Deininger, MWN & Druker, B 2004, 'Catalytic domains of tyrosine kinases determine the phosphorylation sites within c-Cbl', FEBS Letters, vol. 577, no. 3, pp. 555-562. https://doi.org/10.1016/j.febslet.2004.10.054
Grossmann AH, Kolibaba KS, Willis SG, Corbin AS, Langdon WS, Deininger MWN et al. Catalytic domains of tyrosine kinases determine the phosphorylation sites within c-Cbl. FEBS Letters. 2004 Nov 19;577(3):555-562. https://doi.org/10.1016/j.febslet.2004.10.054
Grossmann, A. H. ; Kolibaba, K. S. ; Willis, S. G. ; Corbin, A. S. ; Langdon, W. S. ; Deininger, M. W N ; Druker, Brian. / Catalytic domains of tyrosine kinases determine the phosphorylation sites within c-Cbl. In: FEBS Letters. 2004 ; Vol. 577, No. 3. pp. 555-562.
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