Catabolite control protein a controls hydrogen peroxide production and cell death in streptococcus sanguinis

Lanyan Zheng, Zhijun Chen, Andreas Itzek, Michael Ashby, Jens Kreth

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Streptococcus sanguinis is a commensal oral bacterium producing hydrogen peroxide (H2O2) that is dependent on pyruvate oxidase (Spx) activity. In addition to its well-known role in bacterial antagonism during interspecies competition, H2O2 causes cell death in about 10% of the S. sanguinis population. As a consequence of H 2O2-induced cell death, largely intact chromosomal DNA is released into the environment. This extracellular DNA (eDNA) contributes to the self-aggregation phenotype under aerobic conditions. To further investigate the regulation of spx gene expression, we assessed the role of catabolite control protein A (CcpA) in spx expression control. We report here that CcpA represses spx expression. An isogenic ΔccpA mutant showed elevated spx expression, increased Spx abundance, and H2O2 production, whereas the wild type did not respond with altered spx expression in the presence of glucose and other carbohydrates. Since H2O2 is directly involved in the release of eDNA and bacterial cell death, the presented data suggest that CcpA is a central control element in this important developmental process in S. sanguinis.

Original languageEnglish (US)
Pages (from-to)516-526
Number of pages11
JournalJournal of Bacteriology
Volume193
Issue number2
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Fingerprint

Staphylococcal Protein A
Streptococcus
Hydrogen Peroxide
Cell Death
Pyruvate Oxidase
Bacterial DNA
Proteins
DNA
Gene Expression Regulation
Cause of Death
Carbohydrates
Bacteria
Phenotype
Glucose
Population

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Catabolite control protein a controls hydrogen peroxide production and cell death in streptococcus sanguinis. / Zheng, Lanyan; Chen, Zhijun; Itzek, Andreas; Ashby, Michael; Kreth, Jens.

In: Journal of Bacteriology, Vol. 193, No. 2, 01.01.2011, p. 516-526.

Research output: Contribution to journalArticle

Zheng, Lanyan ; Chen, Zhijun ; Itzek, Andreas ; Ashby, Michael ; Kreth, Jens. / Catabolite control protein a controls hydrogen peroxide production and cell death in streptococcus sanguinis. In: Journal of Bacteriology. 2011 ; Vol. 193, No. 2. pp. 516-526.
@article{ab2aacccf834400f8808872d000a8a5f,
title = "Catabolite control protein a controls hydrogen peroxide production and cell death in streptococcus sanguinis",
abstract = "Streptococcus sanguinis is a commensal oral bacterium producing hydrogen peroxide (H2O2) that is dependent on pyruvate oxidase (Spx) activity. In addition to its well-known role in bacterial antagonism during interspecies competition, H2O2 causes cell death in about 10{\%} of the S. sanguinis population. As a consequence of H 2O2-induced cell death, largely intact chromosomal DNA is released into the environment. This extracellular DNA (eDNA) contributes to the self-aggregation phenotype under aerobic conditions. To further investigate the regulation of spx gene expression, we assessed the role of catabolite control protein A (CcpA) in spx expression control. We report here that CcpA represses spx expression. An isogenic ΔccpA mutant showed elevated spx expression, increased Spx abundance, and H2O2 production, whereas the wild type did not respond with altered spx expression in the presence of glucose and other carbohydrates. Since H2O2 is directly involved in the release of eDNA and bacterial cell death, the presented data suggest that CcpA is a central control element in this important developmental process in S. sanguinis.",
author = "Lanyan Zheng and Zhijun Chen and Andreas Itzek and Michael Ashby and Jens Kreth",
year = "2011",
month = "1",
day = "1",
doi = "10.1128/JB.01131-10",
language = "English (US)",
volume = "193",
pages = "516--526",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Catabolite control protein a controls hydrogen peroxide production and cell death in streptococcus sanguinis

AU - Zheng, Lanyan

AU - Chen, Zhijun

AU - Itzek, Andreas

AU - Ashby, Michael

AU - Kreth, Jens

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Streptococcus sanguinis is a commensal oral bacterium producing hydrogen peroxide (H2O2) that is dependent on pyruvate oxidase (Spx) activity. In addition to its well-known role in bacterial antagonism during interspecies competition, H2O2 causes cell death in about 10% of the S. sanguinis population. As a consequence of H 2O2-induced cell death, largely intact chromosomal DNA is released into the environment. This extracellular DNA (eDNA) contributes to the self-aggregation phenotype under aerobic conditions. To further investigate the regulation of spx gene expression, we assessed the role of catabolite control protein A (CcpA) in spx expression control. We report here that CcpA represses spx expression. An isogenic ΔccpA mutant showed elevated spx expression, increased Spx abundance, and H2O2 production, whereas the wild type did not respond with altered spx expression in the presence of glucose and other carbohydrates. Since H2O2 is directly involved in the release of eDNA and bacterial cell death, the presented data suggest that CcpA is a central control element in this important developmental process in S. sanguinis.

AB - Streptococcus sanguinis is a commensal oral bacterium producing hydrogen peroxide (H2O2) that is dependent on pyruvate oxidase (Spx) activity. In addition to its well-known role in bacterial antagonism during interspecies competition, H2O2 causes cell death in about 10% of the S. sanguinis population. As a consequence of H 2O2-induced cell death, largely intact chromosomal DNA is released into the environment. This extracellular DNA (eDNA) contributes to the self-aggregation phenotype under aerobic conditions. To further investigate the regulation of spx gene expression, we assessed the role of catabolite control protein A (CcpA) in spx expression control. We report here that CcpA represses spx expression. An isogenic ΔccpA mutant showed elevated spx expression, increased Spx abundance, and H2O2 production, whereas the wild type did not respond with altered spx expression in the presence of glucose and other carbohydrates. Since H2O2 is directly involved in the release of eDNA and bacterial cell death, the presented data suggest that CcpA is a central control element in this important developmental process in S. sanguinis.

UR - http://www.scopus.com/inward/record.url?scp=78650856199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650856199&partnerID=8YFLogxK

U2 - 10.1128/JB.01131-10

DO - 10.1128/JB.01131-10

M3 - Article

C2 - 21036992

AN - SCOPUS:78650856199

VL - 193

SP - 516

EP - 526

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 2

ER -