Carbohydrate composition of central nervous system synapses. Analysis of isolated synaptic junctional complexes and postsynaptic densities

Lynn Churchill, Carl Cotman, Gary Banker, Paul Kelly, Leland Shannon

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The composition of specialized structures present at synapses within the central nervous system was elucidated by biochemical analysis of fractions enriched in synaptic junctional complexes and postsynaptic densities. The results indicate that the synaptic junctional complex is primarily protein together with some glycoproteins. The synaptic junctional complex proteins are similar in amino acid composition to synaptic membrane proteins; they are not especially rich in basic residues, as previously suggested. The major carbohydrates present in the synaptic junctional complex and postsynaptic density glycoproteins are mannose, galactose, and glucosamine, with lesser amounts of fucose, N-acetylneuraminic acid, and galactosamine. Comparison with the synaptic membrane fraction indicates that galactose is more concentrated in the synaptic junctional complex and mannose in the postsynaptic density. Glucose is dramatically enriched in both these fractions. Sucrose binding during isolation may partially account for the glucose enrichment.

Original languageEnglish (US)
Pages (from-to)57-72
Number of pages16
JournalBBA - Biomembranes
Volume448
Issue number1
DOIs
StatePublished - Sep 21 1976
Externally publishedYes

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Post-Synaptic Density
Neurology
Mannose
Galactose
Synapses
Synaptic Membranes
Glycoproteins
Central Nervous System
Carbohydrates
Glucose
Galactosamine
Fucose
Glucosamine
N-Acetylneuraminic Acid
Chemical analysis
Sucrose
Membrane Proteins
Proteins
Membranes
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

Carbohydrate composition of central nervous system synapses. Analysis of isolated synaptic junctional complexes and postsynaptic densities. / Churchill, Lynn; Cotman, Carl; Banker, Gary; Kelly, Paul; Shannon, Leland.

In: BBA - Biomembranes, Vol. 448, No. 1, 21.09.1976, p. 57-72.

Research output: Contribution to journalArticle

Churchill, Lynn ; Cotman, Carl ; Banker, Gary ; Kelly, Paul ; Shannon, Leland. / Carbohydrate composition of central nervous system synapses. Analysis of isolated synaptic junctional complexes and postsynaptic densities. In: BBA - Biomembranes. 1976 ; Vol. 448, No. 1. pp. 57-72.
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