TY - JOUR
T1 - Carbohydrate composition of central nervous system synapses. Analysis of isolated synaptic junctional complexes and postsynaptic densities
AU - Churchill, Lynn
AU - Cotman, Carl
AU - Banker, Gary
AU - Kelly, Paul
AU - Shannon, Leland
N1 - Funding Information:
This work was supported by Grant N.I.N.D.S. NS 08597. L.C. and G.B. were supported by predoctoral fellowships from the National Institutes of Mental Health (MH 51697 and MH 50166, respectively). The authors wish to express appreciation to Dr. Jim Partridge for technical advice in the gas chromatographic analysis and to Dr. Wolfgram for the amino acid analysis. We thank Mr. David Shelton for his technical assistance.
PY - 1976/9/21
Y1 - 1976/9/21
N2 - The composition of specialized structures present at synapses within the central nervous system was elucidated by biochemical analysis of fractions enriched in synaptic junctional complexes and postsynaptic densities. The results indicate that the synaptic junctional complex is primarily protein together with some glycoproteins. The synaptic junctional complex proteins are similar in amino acid composition to synaptic membrane proteins; they are not especially rich in basic residues, as previously suggested. The major carbohydrates present in the synaptic junctional complex and postsynaptic density glycoproteins are mannose, galactose, and glucosamine, with lesser amounts of fucose, N-acetylneuraminic acid, and galactosamine. Comparison with the synaptic membrane fraction indicates that galactose is more concentrated in the synaptic junctional complex and mannose in the postsynaptic density. Glucose is dramatically enriched in both these fractions. Sucrose binding during isolation may partially account for the glucose enrichment.
AB - The composition of specialized structures present at synapses within the central nervous system was elucidated by biochemical analysis of fractions enriched in synaptic junctional complexes and postsynaptic densities. The results indicate that the synaptic junctional complex is primarily protein together with some glycoproteins. The synaptic junctional complex proteins are similar in amino acid composition to synaptic membrane proteins; they are not especially rich in basic residues, as previously suggested. The major carbohydrates present in the synaptic junctional complex and postsynaptic density glycoproteins are mannose, galactose, and glucosamine, with lesser amounts of fucose, N-acetylneuraminic acid, and galactosamine. Comparison with the synaptic membrane fraction indicates that galactose is more concentrated in the synaptic junctional complex and mannose in the postsynaptic density. Glucose is dramatically enriched in both these fractions. Sucrose binding during isolation may partially account for the glucose enrichment.
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U2 - 10.1016/0005-2736(76)90076-6
DO - 10.1016/0005-2736(76)90076-6
M3 - Article
C2 - 971428
AN - SCOPUS:0017171295
SN - 0005-2736
VL - 448
SP - 57
EP - 72
JO - BBA - Biomembranes
JF - BBA - Biomembranes
IS - 1
ER -