Carbamoylation correlates of cyanate neuropathy and cyanide poisoning: Relevance to the biomarkers of cassava cyanogenesis and motor system toxicity

Samuel Kimani, Victor Moterroso, Mike Lasarev, Sinei Kipruto, Fred Bukachi, Charles Maitai, Larry David, Daniel Tshala-Katumbay

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We sought to elucidate the protein carbamoylation patterns associated with cyanate neuropathy relative to cyanide poisoning. We hypothesized that under a diet deficient in sulfur amino acids (SAA), the carbamoylation pattern associated with cyanide poisoning is similar to that of cyanate neuropathy. Male rats (6-8 weeks old) were fed a diet with all amino acids (AAA) or 75%-deficiency in SAA and treated with 2.5 mg/kg/body weight (bw) NaCN, or 50 mg/kg/bw NaOCN, or 1 μl/g/bw saline, for up to 6 weeks. Albumin and spinal cord proteins were analyzed using liquid chromatography mass spectrometry (LC-MS/MS). Only NaOCN induced motor deficits with significant levels of carbamoylation. At Day 14, we found a diet-treatment interaction effect on albumin carbamoylation (p = 0.07). At Day 28, no effect was attributed to diet (p = 0.71). Mean number of NaCN-carbamoylated sites on albumin was 47.4% higher relative to vehicle (95% CI:16.7-86.4%). Only NaOCN carbamoylated spinal cord proteins, prominently, under SAA-restricted diet. Proteins targets included myelin basic and proteolipid proteins, neurofilament light and glial fibrillary acidic proteins, and 2', 3' cyclic-nucleotide 3'-phosphodiesterase. Under SAA deficiency, chronic but not acute cyanide toxicity may share biomarkers and pathogenetic similarities with cyanate neuropathy. Prevention of carbamoylation may protect against the neuropathic effects of cyanate.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalSpringerPlus
Volume2
Issue number1
DOIs
StatePublished - 2013

Fingerprint

Cyanates
Manihot
Cyanides
Sulfur Amino Acids
Poisoning
Biomarkers
Diet
Albumins
Body Weight
Spinal Cord
Proteins
2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
Myelin Proteolipid Protein
Myelin Basic Protein
Intermediate Filaments
Glial Fibrillary Acidic Protein
Liquid Chromatography
Mass Spectrometry
Light
Amino Acids

Keywords

  • Carbamoylation
  • Cyanate
  • Cyanide
  • Neuropathy
  • Proteomics

ASJC Scopus subject areas

  • General

Cite this

Carbamoylation correlates of cyanate neuropathy and cyanide poisoning : Relevance to the biomarkers of cassava cyanogenesis and motor system toxicity. / Kimani, Samuel; Moterroso, Victor; Lasarev, Mike; Kipruto, Sinei; Bukachi, Fred; Maitai, Charles; David, Larry; Tshala-Katumbay, Daniel.

In: SpringerPlus, Vol. 2, No. 1, 2013, p. 1-8.

Research output: Contribution to journalArticle

Kimani, Samuel ; Moterroso, Victor ; Lasarev, Mike ; Kipruto, Sinei ; Bukachi, Fred ; Maitai, Charles ; David, Larry ; Tshala-Katumbay, Daniel. / Carbamoylation correlates of cyanate neuropathy and cyanide poisoning : Relevance to the biomarkers of cassava cyanogenesis and motor system toxicity. In: SpringerPlus. 2013 ; Vol. 2, No. 1. pp. 1-8.
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