Capture of flowing endothelial cells using surface-immobilized anti-kinase insert domain receptor antibody

Brandon D. Markway, Owen McCarty, Ulla M. Marzec, David W. Courtman, Stephen R. Hanson, Monica Hinds

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

In humans, self-endothelialization of synthetic grafts is severely limited, but a recent interesting idea is to attract endothelial progenitor cells (EPCs) from peripheral blood onto grafts via antibodies directed at proposed EPC markers. Results with anti-CD34 antibodies have shown some promise, but it is unclear whether CD34 is the best marker for cells with re-endothelializing potential. Much evidence points to kinase insert domain receptor (KDR) as an important indicator of endothelial potential if not a definitive marker. Because KDR is not an adhesion molecule (like CD34), we first demonstrated the ability to use adsorbed and protein G-oriented antibody to this receptor to capture flowing cells onto a solid surface. Using endothelial cells and smooth muscle cells, we show in a model system under low shear rates the ability to selectively capture cells by this receptor. Furthermore, our results indicate that concomitant flow of cells lacking the receptor does not affect the efficiency of capture of KDR+ cells but that orienting the antibody significantly increases the efficiency of capture.

Original languageEnglish (US)
Pages (from-to)97-105
Number of pages9
JournalTissue Engineering - Part C: Methods
Volume14
Issue number2
DOIs
StatePublished - 2008

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Vascular Endothelial Growth Factor Receptor-2
Endothelial cells
Antibodies
Endothelial Cells
Grafts
Transplants
Shear deformation
Muscle
Blood
Adhesion
Cells
Smooth Muscle Myocytes
Anti-Idiotypic Antibodies
Proteins
Molecules

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Capture of flowing endothelial cells using surface-immobilized anti-kinase insert domain receptor antibody. / Markway, Brandon D.; McCarty, Owen; Marzec, Ulla M.; Courtman, David W.; Hanson, Stephen R.; Hinds, Monica.

In: Tissue Engineering - Part C: Methods, Vol. 14, No. 2, 2008, p. 97-105.

Research output: Contribution to journalArticle

Markway, Brandon D. ; McCarty, Owen ; Marzec, Ulla M. ; Courtman, David W. ; Hanson, Stephen R. ; Hinds, Monica. / Capture of flowing endothelial cells using surface-immobilized anti-kinase insert domain receptor antibody. In: Tissue Engineering - Part C: Methods. 2008 ; Vol. 14, No. 2. pp. 97-105.
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