Abstract
Avs valuated the utility of capillary electrophoretic methods for analyzing poly(ethylene glycol)(PEG)-modified granulocyte-colony stimulating factor (G-CSF), a long-acting form of GCSFfor the treatment of cancer therapy-induced neutropenia. Low- and high-molecularweightPEG-G-CSF conjugates prepared with aldehyde-activated PEG-5K and PEG-20K wereseparated by high-performance size-exclusion chromatography (HP-SEC), capillary zone electrophoresis(CZE), and sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE). HPSECshowed low resolution for separating mono- and di-PEG-G-CSFs. SDS-CGE had higherresolution, but required a long analysis and had low peak efficiency. CZE could successfullyseparate both PEG-5K- and PEG-20K-conjugated G-CSFs with a running time of 20 min andhigh peak efficiency. In conclusion, CZE was better than SDS-CGE for separating PEG-G-CSFconjugates and will be useful for PEGylation studies, such as reaction monitoring for optimizationof the PEGylation reaction, and purity and stability tests of PEG-G-CSF.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 491-495 |
| Number of pages | 5 |
| Journal | Archives of Pharmacal Research |
| Volume | 33 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2010 |
| Externally published | Yes |
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Keywords
- Capillary electrophoresis
- Granulocyte-colony stimulating factor
- Pegylation
ASJC Scopus subject areas
- Drug Discovery
- Molecular Medicine
- Organic Chemistry
Cite this
Capillary electrophoretic separation of poly(ethylene glycol)-modified granulocyte-colony stimulating factor. / Lee, Soo-Kyung; Na, Dong Hee.
In: Archives of Pharmacal Research, Vol. 33, No. 3, 03.2010, p. 491-495.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Capillary electrophoretic separation of poly(ethylene glycol)-modified granulocyte-colony stimulating factor
AU - Lee, Soo-Kyung
AU - Na, Dong Hee
PY - 2010/3
Y1 - 2010/3
N2 - Avs valuated the utility of capillary electrophoretic methods for analyzing poly(ethylene glycol)(PEG)-modified granulocyte-colony stimulating factor (G-CSF), a long-acting form of GCSFfor the treatment of cancer therapy-induced neutropenia. Low- and high-molecularweightPEG-G-CSF conjugates prepared with aldehyde-activated PEG-5K and PEG-20K wereseparated by high-performance size-exclusion chromatography (HP-SEC), capillary zone electrophoresis(CZE), and sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE). HPSECshowed low resolution for separating mono- and di-PEG-G-CSFs. SDS-CGE had higherresolution, but required a long analysis and had low peak efficiency. CZE could successfullyseparate both PEG-5K- and PEG-20K-conjugated G-CSFs with a running time of 20 min andhigh peak efficiency. In conclusion, CZE was better than SDS-CGE for separating PEG-G-CSFconjugates and will be useful for PEGylation studies, such as reaction monitoring for optimizationof the PEGylation reaction, and purity and stability tests of PEG-G-CSF.
AB - Avs valuated the utility of capillary electrophoretic methods for analyzing poly(ethylene glycol)(PEG)-modified granulocyte-colony stimulating factor (G-CSF), a long-acting form of GCSFfor the treatment of cancer therapy-induced neutropenia. Low- and high-molecularweightPEG-G-CSF conjugates prepared with aldehyde-activated PEG-5K and PEG-20K wereseparated by high-performance size-exclusion chromatography (HP-SEC), capillary zone electrophoresis(CZE), and sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE). HPSECshowed low resolution for separating mono- and di-PEG-G-CSFs. SDS-CGE had higherresolution, but required a long analysis and had low peak efficiency. CZE could successfullyseparate both PEG-5K- and PEG-20K-conjugated G-CSFs with a running time of 20 min andhigh peak efficiency. In conclusion, CZE was better than SDS-CGE for separating PEG-G-CSFconjugates and will be useful for PEGylation studies, such as reaction monitoring for optimizationof the PEGylation reaction, and purity and stability tests of PEG-G-CSF.
KW - Capillary electrophoresis
KW - Granulocyte-colony stimulating factor
KW - Pegylation
UR - http://www.scopus.com/inward/record.url?scp=77953624277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953624277&partnerID=8YFLogxK
U2 - 10.1007/s12272-010-0320-4
DO - 10.1007/s12272-010-0320-4
M3 - Article
C2 - 20361316
AN - SCOPUS:77953624277
VL - 33
SP - 491
EP - 495
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
SN - 0253-6269
IS - 3
ER -