TY - JOUR
T1 - Capillary electrophoretic separation of poly(ethylene glycol)-modified granulocyte-colony stimulating factor
AU - Lee, Kyung Soo
AU - Na, Dong Hee
N1 - Funding Information:
This work was financially supported by the Ministry of Knowledge Economy (MKE) and Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology.
PY - 2010/3
Y1 - 2010/3
N2 - Avs valuated the utility of capillary electrophoretic methods for analyzing poly(ethylene glycol)(PEG)-modified granulocyte-colony stimulating factor (G-CSF), a long-acting form of GCSFfor the treatment of cancer therapy-induced neutropenia. Low- and high-molecularweightPEG-G-CSF conjugates prepared with aldehyde-activated PEG-5K and PEG-20K wereseparated by high-performance size-exclusion chromatography (HP-SEC), capillary zone electrophoresis(CZE), and sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE). HPSECshowed low resolution for separating mono- and di-PEG-G-CSFs. SDS-CGE had higherresolution, but required a long analysis and had low peak efficiency. CZE could successfullyseparate both PEG-5K- and PEG-20K-conjugated G-CSFs with a running time of 20 min andhigh peak efficiency. In conclusion, CZE was better than SDS-CGE for separating PEG-G-CSFconjugates and will be useful for PEGylation studies, such as reaction monitoring for optimizationof the PEGylation reaction, and purity and stability tests of PEG-G-CSF.
AB - Avs valuated the utility of capillary electrophoretic methods for analyzing poly(ethylene glycol)(PEG)-modified granulocyte-colony stimulating factor (G-CSF), a long-acting form of GCSFfor the treatment of cancer therapy-induced neutropenia. Low- and high-molecularweightPEG-G-CSF conjugates prepared with aldehyde-activated PEG-5K and PEG-20K wereseparated by high-performance size-exclusion chromatography (HP-SEC), capillary zone electrophoresis(CZE), and sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE). HPSECshowed low resolution for separating mono- and di-PEG-G-CSFs. SDS-CGE had higherresolution, but required a long analysis and had low peak efficiency. CZE could successfullyseparate both PEG-5K- and PEG-20K-conjugated G-CSFs with a running time of 20 min andhigh peak efficiency. In conclusion, CZE was better than SDS-CGE for separating PEG-G-CSFconjugates and will be useful for PEGylation studies, such as reaction monitoring for optimizationof the PEGylation reaction, and purity and stability tests of PEG-G-CSF.
KW - Capillary electrophoresis
KW - Granulocyte-colony stimulating factor
KW - Pegylation
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U2 - 10.1007/s12272-010-0320-4
DO - 10.1007/s12272-010-0320-4
M3 - Article
C2 - 20361316
AN - SCOPUS:77953624277
VL - 33
SP - 491
EP - 495
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
SN - 0253-6269
IS - 3
ER -