Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance

Nobuaki Takahashi, Hsing Yu Chen, Isaac S. Harris, Daniel G. Stover, Laura M. Selfors, Roderick T. Bronson, Thomas Deraedt, Karen Cichowski, Alana L. Welm, Yasuo Mori, Gordon Mills, Joan S. Brugge

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cancer cell survival is dependent on oxidative-stress defenses against reactive oxygen species (ROS) that accumulate during tumorigenesis. Here, we show a non-canonical oxidative-stress defense mechanism through TRPA1, a neuronal redox-sensing Ca2+-influx channel. In TRPA1-enriched breast and lung cancer spheroids, TRPA1 is critical for survival of inner cells that exhibit ROS accumulation. Moreover, TRPA1 promotes resistance to ROS-producing chemotherapies, and TRPA1 inhibition suppresses xenograft tumor growth and enhances chemosensitivity. TRPA1 does not affect redox status but upregulates Ca2+-dependent anti-apoptotic pathways. NRF2, an oxidant-defense transcription factor, directly controls TRPA1 expression, thus providing an orthogonal mechanism for protection against oxidative stress together with canonical ROS-neutralizing mechanisms. These findings reveal an oxidative-stress defense program involving TRPA1 that could be exploited for targeted cancer therapies. Takahashi et al. show that TRPA1, a neuronal redox-sensing Ca2+-influx channel overexpressed in human cancer, upregulates Ca2+-dependent anti-apoptotic pathways to promote ROS resistance. NRF2 directly controls TRPA1 expression and TRPA1 inhibition suppresses xenograft tumor growth and enhances chemosensitivity.

Original languageEnglish (US)
JournalCancer Cell
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

Fingerprint

Oxidation-Reduction
Reactive Oxygen Species
Oxidative Stress
Neoplasms
Heterografts
Cell Survival
Up-Regulation
Growth
Oxidants
Lung Neoplasms
Carcinogenesis
Transcription Factors
Breast Neoplasms
Drug Therapy
Therapeutics

Keywords

  • anchorage-independent growth
  • anti-apoptosis
  • Ca signaling
  • chemotherapy resistance
  • NRF2
  • oxidative stress
  • TRP channel
  • TRPA1
  • tumor progression

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

Cite this

Takahashi, N., Chen, H. Y., Harris, I. S., Stover, D. G., Selfors, L. M., Bronson, R. T., ... Brugge, J. S. (Accepted/In press). Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance. Cancer Cell. https://doi.org/10.1016/j.ccell.2018.05.001

Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance. / Takahashi, Nobuaki; Chen, Hsing Yu; Harris, Isaac S.; Stover, Daniel G.; Selfors, Laura M.; Bronson, Roderick T.; Deraedt, Thomas; Cichowski, Karen; Welm, Alana L.; Mori, Yasuo; Mills, Gordon; Brugge, Joan S.

In: Cancer Cell, 01.01.2018.

Research output: Contribution to journalArticle

Takahashi, N, Chen, HY, Harris, IS, Stover, DG, Selfors, LM, Bronson, RT, Deraedt, T, Cichowski, K, Welm, AL, Mori, Y, Mills, G & Brugge, JS 2018, 'Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance', Cancer Cell. https://doi.org/10.1016/j.ccell.2018.05.001
Takahashi, Nobuaki ; Chen, Hsing Yu ; Harris, Isaac S. ; Stover, Daniel G. ; Selfors, Laura M. ; Bronson, Roderick T. ; Deraedt, Thomas ; Cichowski, Karen ; Welm, Alana L. ; Mori, Yasuo ; Mills, Gordon ; Brugge, Joan S. / Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance. In: Cancer Cell. 2018.
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