cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway

Mark R. Vossler, Hong Yao, Randall D. York, Ming Gui Pan, Caroline S. Rim, Philip Stork

Research output: Contribution to journalArticle

904 Citations (Scopus)

Abstract

Cyclic adenosine monophosphate (cAMP) has tissue-specific effects on growth, differentiation, and gene expression. We show here that cAMP can activate the transcription factor Elk-1 and induce neuronal differentiation of PC12 cells via its activation of the MAP kinase cascade. These cell type- specific actions of cAMP require the expression of the serine/threonine kinase B-Raf and activation of the small G protein Rap1. Rap1, activated by mutation or by the cAMP-dependent protein kinase PKA, is a selective activator of B-Raf and an inhibitor of Raf-1. Therefore, in B-Raf-expressing cells, the activation of Rap1 provides a mechanism for tissue-specific regulation of cell growth and differentiation via MAP kinase.

Original languageEnglish (US)
Pages (from-to)73-82
Number of pages10
JournalCell
Volume89
Issue number1
StatePublished - Apr 4 1997

Fingerprint

Cyclic AMP
Phosphotransferases
Chemical activation
ets-Domain Protein Elk-1
Tissue
Monomeric GTP-Binding Proteins
MAP Kinase Signaling System
Protein-Serine-Threonine Kinases
PC12 Cells
Cell growth
Growth
Cyclic AMP-Dependent Protein Kinases
Gene expression
Cell Differentiation
Gene Expression
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Vossler, M. R., Yao, H., York, R. D., Pan, M. G., Rim, C. S., & Stork, P. (1997). cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway. Cell, 89(1), 73-82.

cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway. / Vossler, Mark R.; Yao, Hong; York, Randall D.; Pan, Ming Gui; Rim, Caroline S.; Stork, Philip.

In: Cell, Vol. 89, No. 1, 04.04.1997, p. 73-82.

Research output: Contribution to journalArticle

Vossler, MR, Yao, H, York, RD, Pan, MG, Rim, CS & Stork, P 1997, 'cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway', Cell, vol. 89, no. 1, pp. 73-82.
Vossler MR, Yao H, York RD, Pan MG, Rim CS, Stork P. cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway. Cell. 1997 Apr 4;89(1):73-82.
Vossler, Mark R. ; Yao, Hong ; York, Randall D. ; Pan, Ming Gui ; Rim, Caroline S. ; Stork, Philip. / cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway. In: Cell. 1997 ; Vol. 89, No. 1. pp. 73-82.
@article{f03950790861475e94aae979b9beaed4,
title = "cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway",
abstract = "Cyclic adenosine monophosphate (cAMP) has tissue-specific effects on growth, differentiation, and gene expression. We show here that cAMP can activate the transcription factor Elk-1 and induce neuronal differentiation of PC12 cells via its activation of the MAP kinase cascade. These cell type- specific actions of cAMP require the expression of the serine/threonine kinase B-Raf and activation of the small G protein Rap1. Rap1, activated by mutation or by the cAMP-dependent protein kinase PKA, is a selective activator of B-Raf and an inhibitor of Raf-1. Therefore, in B-Raf-expressing cells, the activation of Rap1 provides a mechanism for tissue-specific regulation of cell growth and differentiation via MAP kinase.",
author = "Vossler, {Mark R.} and Hong Yao and York, {Randall D.} and Pan, {Ming Gui} and Rim, {Caroline S.} and Philip Stork",
year = "1997",
month = "4",
day = "4",
language = "English (US)",
volume = "89",
pages = "73--82",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - cAMP activates MAP kinase and Elk-1 through a B-Raf- and Rap1-dependent pathway

AU - Vossler, Mark R.

AU - Yao, Hong

AU - York, Randall D.

AU - Pan, Ming Gui

AU - Rim, Caroline S.

AU - Stork, Philip

PY - 1997/4/4

Y1 - 1997/4/4

N2 - Cyclic adenosine monophosphate (cAMP) has tissue-specific effects on growth, differentiation, and gene expression. We show here that cAMP can activate the transcription factor Elk-1 and induce neuronal differentiation of PC12 cells via its activation of the MAP kinase cascade. These cell type- specific actions of cAMP require the expression of the serine/threonine kinase B-Raf and activation of the small G protein Rap1. Rap1, activated by mutation or by the cAMP-dependent protein kinase PKA, is a selective activator of B-Raf and an inhibitor of Raf-1. Therefore, in B-Raf-expressing cells, the activation of Rap1 provides a mechanism for tissue-specific regulation of cell growth and differentiation via MAP kinase.

AB - Cyclic adenosine monophosphate (cAMP) has tissue-specific effects on growth, differentiation, and gene expression. We show here that cAMP can activate the transcription factor Elk-1 and induce neuronal differentiation of PC12 cells via its activation of the MAP kinase cascade. These cell type- specific actions of cAMP require the expression of the serine/threonine kinase B-Raf and activation of the small G protein Rap1. Rap1, activated by mutation or by the cAMP-dependent protein kinase PKA, is a selective activator of B-Raf and an inhibitor of Raf-1. Therefore, in B-Raf-expressing cells, the activation of Rap1 provides a mechanism for tissue-specific regulation of cell growth and differentiation via MAP kinase.

UR - http://www.scopus.com/inward/record.url?scp=0030963439&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030963439&partnerID=8YFLogxK

M3 - Article

VL - 89

SP - 73

EP - 82

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -