Calpain in cultured bovine Lens epithelial cells

Ruth D. Lipman, Deanna E. Cyr, Larry L. David, Allen Taylor

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Calcium dependent proteolysis was examined in supernatant prepared from cultured bovine lens epithelial (BLE) cells. The presence of the calcium activated protease, calpain, was indicated by immunorecognition of 80 kDa and 30 kDa subunits of calpain in BLE cell supernatant. Degradation of 125I-alpha-crystallin and FTTC labeled casein by BLE cell supernatant were shown to be calcium dependent. Inhibition of activity was achieved with EGTA, calpastatin or CbzValPheH. The data presented are the first measurement of calpain activity in cultured lens cells.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalCurrent Eye Research
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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