Calpain II in two in vivo models of sugar cataract

Mitsuyoshi Azuma, Thomas (Tom) Shearer, Takahiro Matsumoto, Larry David, Takashi Murach

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Cataracts were produced in rat lenses by either feeding a diet containing 50% galactose or by inducing diabetic condition by intravenous injection of streptozotocin. Proteolysis of crystallins, protease activity of calpain II enzyme (EC 3.4.22.17), and presence of calpain molecule (antigen) were determined at four cataract stages-I, cortical vacuoles, II, vacuoles plus hazy cortex, III, nuclear cataract, and IV, mature cataracts. Calpain activity was normal or moderately elevated at early stages of galactose and diabetic cataracts. Later stages III and IV showed proteolysis of lens crystallins, increased proportion of insoluble proteins, loss of calpain enzyme activity and calpain molecule from the soluble fraction, and reduced amounts of calpain associated with insoluble pellet. In galactose cataract, the largest increase in lens calcium were found when proteolysis was present. These results provide evidence for calpain-induced proteolysis of lens crystallins in two in vivo models of sugar cataracts in rodents.

Original languageEnglish (US)
Pages (from-to)393-401
Number of pages9
JournalExperimental Eye Research
Volume51
Issue number4
DOIs
StatePublished - 1990

Fingerprint

Calpain
Cataract
Crystallins
Lenses
Proteolysis
Galactose
Vacuoles
Enzymes
Streptozocin
Intravenous Injections
Rodentia
Peptide Hydrolases
Diet
Calcium
Antigens

Keywords

  • calpain
  • diabetic cataracts
  • galactose cataracts
  • proteolysis
  • rat

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Calpain II in two in vivo models of sugar cataract. / Azuma, Mitsuyoshi; Shearer, Thomas (Tom); Matsumoto, Takahiro; David, Larry; Murach, Takashi.

In: Experimental Eye Research, Vol. 51, No. 4, 1990, p. 393-401.

Research output: Contribution to journalArticle

Azuma, Mitsuyoshi ; Shearer, Thomas (Tom) ; Matsumoto, Takahiro ; David, Larry ; Murach, Takashi. / Calpain II in two in vivo models of sugar cataract. In: Experimental Eye Research. 1990 ; Vol. 51, No. 4. pp. 393-401.
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