Calmodulin-dependent protein kinases purified from rat brain and rabbit liver

Charles M. Schworer, Robert W. McClure, Thomas Soderling

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A calmodulin-dependent protein kinase was purified from rat brain by the same protocol used previously for a rabbit liver calmodulin-dependent glycogen synthase kinase. The rat brain kinase readily phosphorylated rabbit skeletal muscle glycogen synthase at sites 1b and 2, the same sites phosphorylated by rabbit liver calmodulin-dependent kinase. The two kinases have other similarities: substrate specificity, potent inhibition by sodium fluoride, and nearly equal Ka's (10-20 nm) for calmodulin. Also, both enzymes have similar Stokes radii, 70 Å (rabbit liver) and 75 Å (rat brain), but quite different sedimentation coefficients, 10.6 S and 17.4 S, respectively. Consequently, the calculated molecular weights are also different: 560,000 for the brain enzyme and 300,000 for the liver enzyme. The major subunit of the rat brain kinase appears to be a single 51-kDa peptide, not a doublet pattern of 51- and 53-kDa subunits that is characteristic of the rabbit liver enzyme. Our findings are consistent with the hypothesis that the rat brain and rabbit liver enzymes belong to a class of closely related calmodulin-dependent protein kinases, possibly isozymes. This class of enzymes may be responsible for regulating several of the known calcium-dependent physiological functions.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume242
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

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Calcium-Calmodulin-Dependent Protein Kinases
Liver
Rats
Brain
Rabbits
Phosphotransferases
Enzymes
Calmodulin
Glycogen Synthase Kinases
Glycogen Synthase
Sodium Fluoride
Substrate Specificity
Sedimentation
Isoenzymes
Muscle
Skeletal Muscle
Molecular Weight
Molecular weight
Calcium
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Calmodulin-dependent protein kinases purified from rat brain and rabbit liver. / Schworer, Charles M.; McClure, Robert W.; Soderling, Thomas.

In: Archives of Biochemistry and Biophysics, Vol. 242, No. 1, 1985, p. 137-145.

Research output: Contribution to journalArticle

Schworer, Charles M. ; McClure, Robert W. ; Soderling, Thomas. / Calmodulin-dependent protein kinases purified from rat brain and rabbit liver. In: Archives of Biochemistry and Biophysics. 1985 ; Vol. 242, No. 1. pp. 137-145.
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