Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors

Melinda J. Walters, Gary A. Wayman, John C. Notis, Richard Goodman, Thomas Soderling, Jan L. Christian

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In the current study, we show that bone morphogenetic proteins (BMPs) play a role in hematopoiesis that is independent of their function in specifying ventral mesodermal fate. When BMP activity is upregulated or inhibited in Xenopus embryos hematopoietic precursors are specified properly but few mature erythrocytes are generated. Distinct cellular defects underlie this loss of erythrocytes: inhibition of BMP activity induces erythroid precursors to undergo apoptotic cell death, whereas constitutive activation of BMPs causes an increase in commitment of hematopoietic progenitors to myeloid differentiation and a concomitant decrease in erythrocytes that is not due to enhanced apoptosis. These blood defects are observed even when BMP activity is misregulated solely in non-hematopoietic (ectodermal) cells, demonstrating that BMPs generate extrinsic signals that regulate hematopoiesis independent of mesodermal patterning. Further analysis revealed that endogenous calmodulin-dependent protein kinase IV (CaM KIV) is required to negatively modulate hematopoietic functions of BMPs downstream of receptor activation. Our data are consistent with a model in which CaM KIV inhibits BMP signals by activating a substrate, possibly cAMP-response element-binding protein (CREB), that recruits limiting amounts of CREB binding protein (CBP) away from transcriptional complexes functioning downstream of BMPs.

Original languageEnglish (US)
Pages (from-to)1455-1466
Number of pages12
JournalDevelopment
Volume129
Issue number6
StatePublished - 2002

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Calcium-Calmodulin-Dependent Protein Kinase Type 4
Bone Morphogenetic Proteins
Cyclic AMP Response Element-Binding Protein
Erythrocytes
Hematopoiesis
Bone Morphogenetic Protein Receptors
Xenopus
Protein Binding
Carrier Proteins
Cell Death
Embryonic Structures
Apoptosis

Keywords

  • BMPs
  • CaM KIV
  • CBP
  • CREB
  • Hematopoiesis
  • Xenopus laevis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Walters, M. J., Wayman, G. A., Notis, J. C., Goodman, R., Soderling, T., & Christian, J. L. (2002). Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors. Development, 129(6), 1455-1466.

Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors. / Walters, Melinda J.; Wayman, Gary A.; Notis, John C.; Goodman, Richard; Soderling, Thomas; Christian, Jan L.

In: Development, Vol. 129, No. 6, 2002, p. 1455-1466.

Research output: Contribution to journalArticle

Walters, MJ, Wayman, GA, Notis, JC, Goodman, R, Soderling, T & Christian, JL 2002, 'Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors', Development, vol. 129, no. 6, pp. 1455-1466.
Walters, Melinda J. ; Wayman, Gary A. ; Notis, John C. ; Goodman, Richard ; Soderling, Thomas ; Christian, Jan L. / Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors. In: Development. 2002 ; Vol. 129, No. 6. pp. 1455-1466.
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