Calmodulin-dependent glycogen synthase kinase

Identification in liver of normal and phosphorylase kinase-deficient rats

Charles M. Schworer, M. Elizabeth Payne, Alan T. Williams, Thomas Soderling

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have purified a calmodulin-dependent glycogen synthase kinase from livers of normal and phosphorylase kinase-deficient (gsd/gsd) rats. No differences between normal and gsd/gsd rats were apparent in either (a) the ability of liver extracts to phosphorylate exogenous glycogen synthase in a Ca2+- and calmodulin-dependent manner or (b) the purification of the calmodulin-dependent synthase kinase. Although extracts from rat liver, when compared to rabbit liver extracts, had a significantly reduced ability to phosphorylate exogenous synthase, the calmodulin-dependent synthase kinase could be purified from rat liver using a protocol identical to that described for rabbit liver. Moreover, the synthase kinase purified from rat liver had properties very similar to those of the rabbit liver enzyme. The enzyme was completely dependent on calmodulin for activity against glycogen synthase, was unable to phosphorylate phosphorylase b, catalyzed the rapid incorporation of 0.4 mol phosphate/mol of glycogen synthase subunit, selectively phosphorylated sites 1b and 2 in the glycogen synthase molecule, had a Stokes' radius of about 70 Å, and appeared to be composed of subunits of Mr 56,000 and 57,000. These observations led us to conclude that (1) calmodulin-dependent glycogen synthase kinase is distinct from other kinases previously described and (2) the rat liver kinase and the rabbit liver kinase are very similar enzymes.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume224
Issue number1
DOIs
StatePublished - Jul 1 1983
Externally publishedYes

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Phosphorylase Kinase
Glycogen Synthase Kinases
Calmodulin
Liver
Rats
Glycogen Synthase
Phosphotransferases
Liver Extracts
Rabbits
Enzymes
Phosphorylase b
Purification
Phosphates
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Calmodulin-dependent glycogen synthase kinase : Identification in liver of normal and phosphorylase kinase-deficient rats. / Schworer, Charles M.; Payne, M. Elizabeth; Williams, Alan T.; Soderling, Thomas.

In: Archives of Biochemistry and Biophysics, Vol. 224, No. 1, 01.07.1983, p. 77-86.

Research output: Contribution to journalArticle

Schworer, Charles M. ; Payne, M. Elizabeth ; Williams, Alan T. ; Soderling, Thomas. / Calmodulin-dependent glycogen synthase kinase : Identification in liver of normal and phosphorylase kinase-deficient rats. In: Archives of Biochemistry and Biophysics. 1983 ; Vol. 224, No. 1. pp. 77-86.
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