Calibration of optical coherence tomography angiography with a microfluidic chip

Johnny P. Su, Rahul Chandwani, Simon S. Gao, Alex D. Pechauer, Miao Zhang, Jie Wang, Jia Yali, David Huang, Gangjun Liu

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A microfluidic chip with microchannels ranging from 8 to 96 μm was used to mimic blood vessels down to the capillary level. Blood flow within the microfluidic channels was analyzed with split-spectrum amplitude-decorrelation angiography (SSADA)-based optical coherence tomography (OCT) angiography. It was found that the SSADA decorrelation value was related to both blood flow speed and channel width. SSADA could differentiate nonflowing blood inside the microfluidic channels from static paper. The SSADA decorrelation value was approximately linear with blood flow velocity up to a threshold Vsat of 5.83±1.33 mm/s (mean±standard deviation over the range of channel widths). Beyond this threshold, it approached a saturation value Dsat. Dsat was higher for wider channels, and approached a maximum value Dsm as the channel width became much larger than the beam focal spot diameter. These results indicate that decorrelation values (flow signal) in capillary networks would be proportional to both flow velocity and vessel caliber but would be capped at a saturation value in larger blood vessels. These findings are useful for interpretation and quantification of clinical OCT angiography results.

Original languageEnglish (US)
Article number086015
JournalJournal of Biomedical Optics
Volume21
Issue number8
DOIs
StatePublished - Aug 1 2016

Keywords

  • medical and biological imaging
  • microfluidic device
  • ophthalmic optics and devices
  • optical coherence tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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  • Cite this

    Su, J. P., Chandwani, R., Gao, S. S., Pechauer, A. D., Zhang, M., Wang, J., Yali, J., Huang, D., & Liu, G. (2016). Calibration of optical coherence tomography angiography with a microfluidic chip. Journal of Biomedical Optics, 21(8), [086015]. https://doi.org/10.1117/1.JBO.21.8.086015