TY - JOUR
T1 - Calculated and experimental spin state of seleno cytochrome p450
AU - Jiang, Yongying
AU - Sivaramakrishnan, Santhosh
AU - Hayashi, Takahiro
AU - Cohen, Shimrit
AU - Moënneloccoz, Pierre
AU - Shaik, Sason
AU - De Ortiz Montellano, Paul R.
PY - 2009/9/14
Y1 - 2009/9/14
N2 - A change for the better: The proximal cysteine thiolate in a cytochrome P450 enzyme is replaced by a selenocysteine. The resulting selenolate-ligated CYP119 protein has UV/Vis (see spectrum, ), EPR, and resonance Raman spectra that are similar to those of the native protein ( - ). These results are the first to fully describe a hemoprotein with a selenolate proximal ligand.
AB - A change for the better: The proximal cysteine thiolate in a cytochrome P450 enzyme is replaced by a selenocysteine. The resulting selenolate-ligated CYP119 protein has UV/Vis (see spectrum, ), EPR, and resonance Raman spectra that are similar to those of the native protein ( - ). These results are the first to fully describe a hemoprotein with a selenolate proximal ligand.
KW - Cytochrome p450
KW - Proteins
KW - Raman spectroscopy
KW - Selenium
KW - Spin states
UR - http://www.scopus.com/inward/record.url?scp=70349924985&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349924985&partnerID=8YFLogxK
U2 - 10.1002/anie.200901485
DO - 10.1002/anie.200901485
M3 - Article
C2 - 19718734
AN - SCOPUS:70349924985
SN - 1433-7851
VL - 48
SP - 7193
EP - 7195
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 39
ER -