Calcium/Calmodulin‐Dependent Protein Kinase II in Squid Synaptosomes

Martha Bass, Harish C. Pant, Harold Gainer, Thomas R. Soderling

    Research output: Contribution to journalArticle

    8 Scopus citations

    Abstract

    Abstract: The Ca2+/calmodulin (CaM)‐dependent protein kinase II system in squid nervous tissue was investigated. The Ca2+/CaM‐dependent protein kinase II was found to be very active in the synaptosome preparation from optic lobe, where it was associated with the high‐speed particulate fraction. Incubation of the synaptosomal homogenate with calcium, calmodulin, magnesium, and ATP resulted in partial and reversible conversion of the Ca2+/CaM‐dependent protein kinase II from its calcium‐dependent form to a calcium‐independent species. The magnitude of this conversion reaction could be increased by inclusion of the protein phosphatase inhibitor NaF or by substitution of adenosine 5′‐O‐(3‐thiotriphosphate) for ATP. When [γ‐32P] ATP was used, proteins of 54 and 58 kilodaltons (kDa) as well as proteins >100 kDa were rapidly 32P‐labeled in a calcium‐dependent manner. Major 125I‐CaM binding proteins in the synaptosome membrane fraction were 38 and 54 kDa. The Ca2+/CaM‐dependent protein kinase II was purified from the squid synaptosome and was shown to consist of 54‐and 58–60‐kDa subunits. The purified kinase, like Ca2+/CaM‐dependent protein kinase II from rat brain, catalyzed auto‐phosphorylation associated with formation of the calcium‐independent form. These studies, characterizing the Ca2+/ CaM‐dependent protein kinase II in squid neural tissue, are supportive of the putative role of this kinase in regulating calcium‐dependent synaptic functions.

    Original languageEnglish (US)
    Pages (from-to)1116-1123
    Number of pages8
    JournalJournal of neurochemistry
    Volume49
    Issue number4
    DOIs
    StatePublished - Oct 1987

    Keywords

    • Ca/ calmodulin‐dependent protein kinase II
    • Squid
    • Synaptosomes

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Fingerprint Dive into the research topics of 'Calcium/Calmodulin‐Dependent Protein Kinase II in Squid Synaptosomes'. Together they form a unique fingerprint.

  • Cite this