Calcium waves propagate through radial glial cells and modulate proliferation in the developing neocortex

Tamily A. Weissman, Patricio Riquelme, Lidija Ivic, Alexander C. Flint, Arnold R. Kriegstein

Research output: Contribution to journalArticle

353 Citations (Scopus)

Abstract

The majority of neurons in the adult neocortex are produced embryonically during a brief but intense period of neuronal proliferation. The radial glial cell, a transient embryonic cell type known for its crucial role in neuronal migration, has recently been shown to function as a neuronal progenitor cell and appears to produce most cortical pyramidal neurons. Radial glial cell modulation could thus affect neuron production, neuronal migration, and overall cortical architecture; however, signaling mechanisms among radial glia have not been studied directly. We demonstrate here that calcium waves propagate through radial glial cells in the proliferative cortical ventricular zone (VZ). Radial glial calcium waves occur spontaneously and require connexin hemichannels, P2Y 1 ATP receptors, and intracellular IP 3-mediated calcium release. Furthermore, we show that wave disruption decreases VZ proliferation during the peak of embryonic neurogenesis. Taken together, these results demonstrate a radial glial signaling mechanism that may regulate cortical neuronal production.

Original languageEnglish (US)
Pages (from-to)647-661
Number of pages15
JournalNeuron
Volume43
Issue number5
DOIs
StatePublished - Sep 2 2004
Externally publishedYes

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Ependymoglial Cells
Calcium Signaling
Neocortex
Neuroglia
Cell Proliferation
Purinergic P2 Receptors
Neurons
Connexins
Pyramidal Cells
Neurogenesis
Stem Cells
Calcium

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Calcium waves propagate through radial glial cells and modulate proliferation in the developing neocortex. / Weissman, Tamily A.; Riquelme, Patricio; Ivic, Lidija; Flint, Alexander C.; Kriegstein, Arnold R.

In: Neuron, Vol. 43, No. 5, 02.09.2004, p. 647-661.

Research output: Contribution to journalArticle

Weissman, TA, Riquelme, P, Ivic, L, Flint, AC & Kriegstein, AR 2004, 'Calcium waves propagate through radial glial cells and modulate proliferation in the developing neocortex', Neuron, vol. 43, no. 5, pp. 647-661. https://doi.org/10.1016/j.neuron.2004.08.015
Weissman, Tamily A. ; Riquelme, Patricio ; Ivic, Lidija ; Flint, Alexander C. ; Kriegstein, Arnold R. / Calcium waves propagate through radial glial cells and modulate proliferation in the developing neocortex. In: Neuron. 2004 ; Vol. 43, No. 5. pp. 647-661.
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