Calcium-triggered exocytosis and endocytosis in an isolated presynaptic cell

Capacitance measurements in saccular hair cells

Thomas D. Parsons, David Lenzi, Wolfhard Almers, William M. Roberts

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

Depolarization of isolated frog saccular hair cells caused Ca2+-dependent increases in membrane capacitance that we interpret as the fusion of synaptic vesicles with the plasma membrane. During a maintained depolarization to -10 mV, the capacitance increased at a rate corresponding to the fusion of ∼500 vesicles per second at each active zone. Release continued at this high rate for up to 2 s, long enough to exhaust >5 times the number of vesicles initially in close apposition to the plasma membrane at active zones. We therefore propose that hair cells are specialized for rapid replenishment of vesicles at release sites. Upon repolarization to -70 mV, the capacitance returned exponentially (time constant, ∼14 s) to near the prestimulus level in perforated-patch recordings, but not in whole-cell recordings, suggesting that a mobile intracellular factor is required for membrane retrieval.

Original languageEnglish (US)
Pages (from-to)875-883
Number of pages9
JournalNeuron
Volume13
Issue number4
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Exocytosis
Endocytosis
Cell Membrane
Calcium
Membranes
Synaptic Vesicles
Patch-Clamp Techniques
Anura

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Calcium-triggered exocytosis and endocytosis in an isolated presynaptic cell : Capacitance measurements in saccular hair cells. / Parsons, Thomas D.; Lenzi, David; Almers, Wolfhard; Roberts, William M.

In: Neuron, Vol. 13, No. 4, 1994, p. 875-883.

Research output: Contribution to journalArticle

Parsons, Thomas D. ; Lenzi, David ; Almers, Wolfhard ; Roberts, William M. / Calcium-triggered exocytosis and endocytosis in an isolated presynaptic cell : Capacitance measurements in saccular hair cells. In: Neuron. 1994 ; Vol. 13, No. 4. pp. 875-883.
@article{e8b22cc3e6f14dfda2af459bcd362ac3,
title = "Calcium-triggered exocytosis and endocytosis in an isolated presynaptic cell: Capacitance measurements in saccular hair cells",
abstract = "Depolarization of isolated frog saccular hair cells caused Ca2+-dependent increases in membrane capacitance that we interpret as the fusion of synaptic vesicles with the plasma membrane. During a maintained depolarization to -10 mV, the capacitance increased at a rate corresponding to the fusion of ∼500 vesicles per second at each active zone. Release continued at this high rate for up to 2 s, long enough to exhaust >5 times the number of vesicles initially in close apposition to the plasma membrane at active zones. We therefore propose that hair cells are specialized for rapid replenishment of vesicles at release sites. Upon repolarization to -70 mV, the capacitance returned exponentially (time constant, ∼14 s) to near the prestimulus level in perforated-patch recordings, but not in whole-cell recordings, suggesting that a mobile intracellular factor is required for membrane retrieval.",
author = "Parsons, {Thomas D.} and David Lenzi and Wolfhard Almers and Roberts, {William M.}",
year = "1994",
doi = "10.1016/0896-6273(94)90253-4",
language = "English (US)",
volume = "13",
pages = "875--883",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Calcium-triggered exocytosis and endocytosis in an isolated presynaptic cell

T2 - Capacitance measurements in saccular hair cells

AU - Parsons, Thomas D.

AU - Lenzi, David

AU - Almers, Wolfhard

AU - Roberts, William M.

PY - 1994

Y1 - 1994

N2 - Depolarization of isolated frog saccular hair cells caused Ca2+-dependent increases in membrane capacitance that we interpret as the fusion of synaptic vesicles with the plasma membrane. During a maintained depolarization to -10 mV, the capacitance increased at a rate corresponding to the fusion of ∼500 vesicles per second at each active zone. Release continued at this high rate for up to 2 s, long enough to exhaust >5 times the number of vesicles initially in close apposition to the plasma membrane at active zones. We therefore propose that hair cells are specialized for rapid replenishment of vesicles at release sites. Upon repolarization to -70 mV, the capacitance returned exponentially (time constant, ∼14 s) to near the prestimulus level in perforated-patch recordings, but not in whole-cell recordings, suggesting that a mobile intracellular factor is required for membrane retrieval.

AB - Depolarization of isolated frog saccular hair cells caused Ca2+-dependent increases in membrane capacitance that we interpret as the fusion of synaptic vesicles with the plasma membrane. During a maintained depolarization to -10 mV, the capacitance increased at a rate corresponding to the fusion of ∼500 vesicles per second at each active zone. Release continued at this high rate for up to 2 s, long enough to exhaust >5 times the number of vesicles initially in close apposition to the plasma membrane at active zones. We therefore propose that hair cells are specialized for rapid replenishment of vesicles at release sites. Upon repolarization to -70 mV, the capacitance returned exponentially (time constant, ∼14 s) to near the prestimulus level in perforated-patch recordings, but not in whole-cell recordings, suggesting that a mobile intracellular factor is required for membrane retrieval.

UR - http://www.scopus.com/inward/record.url?scp=0028113779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028113779&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(94)90253-4

DO - 10.1016/0896-6273(94)90253-4

M3 - Article

VL - 13

SP - 875

EP - 883

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 4

ER -