Calcium entry leads to inactivation of calcium channel in Paramecium

Paul Brehm, Roger Eckert

Research output: Contribution to journalArticle

317 Citations (Scopus)

Abstract

Under depolarizing voltage clamp of Paramecium an inward calcium current developed and subsequently relaxed within 10 milliseconds. The relaxation was substantially slowed when most of the extracellular calcium was replaced by either strontium or barium. Evidence is presented that the relaxation is not accounted for by a drop in electromotive force acting on calcium, or by activation of a delayed potassium current. Relaxation of the current must, therefore, result from an inactivation of the calcium channel. This inactivation persisted after a pulse, as manifested by a reduced calcium current during subsequent depolarization. Inactivation was retarded by procedures that reduce net entry of calcium, and was independent of membrane potential. The calcium channel undergoes inactivation as a consequence of calcium entry during depolarization. In this respect, inactivation of the calcium channel departs qualitatively from the behavior described in the Hodgkin-Huxley model of the sodium channel.

Original languageEnglish (US)
Pages (from-to)1203-1206
Number of pages4
JournalScience
Volume202
Issue number4373
StatePublished - 1978
Externally publishedYes

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Paramecium
Calcium Channels
Calcium
Strontium
Sodium Channels
Barium
Membrane Potentials
Potassium

ASJC Scopus subject areas

  • General

Cite this

Calcium entry leads to inactivation of calcium channel in Paramecium. / Brehm, Paul; Eckert, Roger.

In: Science, Vol. 202, No. 4373, 1978, p. 1203-1206.

Research output: Contribution to journalArticle

Brehm, P & Eckert, R 1978, 'Calcium entry leads to inactivation of calcium channel in Paramecium', Science, vol. 202, no. 4373, pp. 1203-1206.
Brehm, Paul ; Eckert, Roger. / Calcium entry leads to inactivation of calcium channel in Paramecium. In: Science. 1978 ; Vol. 202, No. 4373. pp. 1203-1206.
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abstract = "Under depolarizing voltage clamp of Paramecium an inward calcium current developed and subsequently relaxed within 10 milliseconds. The relaxation was substantially slowed when most of the extracellular calcium was replaced by either strontium or barium. Evidence is presented that the relaxation is not accounted for by a drop in electromotive force acting on calcium, or by activation of a delayed potassium current. Relaxation of the current must, therefore, result from an inactivation of the calcium channel. This inactivation persisted after a pulse, as manifested by a reduced calcium current during subsequent depolarization. Inactivation was retarded by procedures that reduce net entry of calcium, and was independent of membrane potential. The calcium channel undergoes inactivation as a consequence of calcium entry during depolarization. In this respect, inactivation of the calcium channel departs qualitatively from the behavior described in the Hodgkin-Huxley model of the sodium channel.",
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AB - Under depolarizing voltage clamp of Paramecium an inward calcium current developed and subsequently relaxed within 10 milliseconds. The relaxation was substantially slowed when most of the extracellular calcium was replaced by either strontium or barium. Evidence is presented that the relaxation is not accounted for by a drop in electromotive force acting on calcium, or by activation of a delayed potassium current. Relaxation of the current must, therefore, result from an inactivation of the calcium channel. This inactivation persisted after a pulse, as manifested by a reduced calcium current during subsequent depolarization. Inactivation was retarded by procedures that reduce net entry of calcium, and was independent of membrane potential. The calcium channel undergoes inactivation as a consequence of calcium entry during depolarization. In this respect, inactivation of the calcium channel departs qualitatively from the behavior described in the Hodgkin-Huxley model of the sodium channel.

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