Calcium-dependent regulation of glycogen synthase activity in a muscle glycogen particle

Ashok K. Srivastava, Balwant S. Khatra, Thomas Soderling

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The calcium-dependent inactivation of glycogen synthase in an isolated glycogen-protein complex (glycogen pellet) from rabbit skeletal muscle has been investigated. Addition of 1 mm Ca2+, 10 mm Mg2+, and 1 mm ATP-γ-S to a concentrated suspension of glycogen pellet resulted in a rapid activation of glycogen phosphorylase concomitant with an inactivation of glycogen synthase. These conversion reactions were blocked by ethylene glycol bis(β-aminoethyl ether) N, N′-tetraacetic acid or by pretreatment of the complex with an antiserum to purified phosphorylase kinase. These data suggest that in the glycogen-protein complex, which may be a functional unit of glycogen metabolism in vivo, phosphorylase kinase can catalyze a Ca2+-dependent activation of glycogen phosphorylase synchronized with an inactivation of glycogen synthase. If under similar conditions phosphoprotein phosphatase activity was assayed using exogenous [32P]phosphorylase, there was an apparent inactivation of the phosphatase. Evidence is presented that this apparent inactivation of phosphatase was due to an accumulation of endogenous phosphorylase a which acted as an inhibitor to the exogenous [32P]-phosphorylase.

Original languageEnglish (US)
Pages (from-to)291-296
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume205
Issue number2
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

Glycogen Synthase
Glycogen
Muscle
Calcium
Muscles
Phosphorylase Kinase
Glycogen Phosphorylase
Phosphorylases
Phosphoric Monoester Hydrolases
Chemical activation
Phosphorylase a
Conversion Disorder
Ethylene Glycol
Phosphoprotein Phosphatases
Metabolism
Ether
Immune Sera
Suspensions
Skeletal Muscle
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Calcium-dependent regulation of glycogen synthase activity in a muscle glycogen particle. / Srivastava, Ashok K.; Khatra, Balwant S.; Soderling, Thomas.

In: Archives of Biochemistry and Biophysics, Vol. 205, No. 2, 1980, p. 291-296.

Research output: Contribution to journalArticle

Srivastava, Ashok K. ; Khatra, Balwant S. ; Soderling, Thomas. / Calcium-dependent regulation of glycogen synthase activity in a muscle glycogen particle. In: Archives of Biochemistry and Biophysics. 1980 ; Vol. 205, No. 2. pp. 291-296.
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