Calcium-dependent polymerization of lactoferrin

Robert M. Bennett; Grover C. Bagby; Julianne Davis

doi:10.1016/S0006-291X(81)80014-9

Calcium-dependent polymerization of lactoferrin

Robert M. Bennett, Grover C. Bagby, Julianne Davis

Hematology & Medical Oncology

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Lactoferrin is known to have a molecular weight of approximately 77,000. In this study, we show that it undergoes polymerization in calcium-containing fluids and that the predominant species is a tetramer. Preliminary evidence is presented to indicate that the tetramer is also found in human serum, tears and breast milk. When the lactoferrin monomer and the tetramer were tested for biological activity in a system that is known to be highly sensitive to an inhibitory effect of lactoferrin - the production of granulocyte monocyte colony-stimulating activity - it was found that only the monomeric form of lactoferrin was inhibitory.

Original language	English (US)
Pages (from-to)	88-95
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	101
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(81)80014-9
State	Published - Jul 16 1981

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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N2 - Lactoferrin is known to have a molecular weight of approximately 77,000. In this study, we show that it undergoes polymerization in calcium-containing fluids and that the predominant species is a tetramer. Preliminary evidence is presented to indicate that the tetramer is also found in human serum, tears and breast milk. When the lactoferrin monomer and the tetramer were tested for biological activity in a system that is known to be highly sensitive to an inhibitory effect of lactoferrin - the production of granulocyte monocyte colony-stimulating activity - it was found that only the monomeric form of lactoferrin was inhibitory.

AB - Lactoferrin is known to have a molecular weight of approximately 77,000. In this study, we show that it undergoes polymerization in calcium-containing fluids and that the predominant species is a tetramer. Preliminary evidence is presented to indicate that the tetramer is also found in human serum, tears and breast milk. When the lactoferrin monomer and the tetramer were tested for biological activity in a system that is known to be highly sensitive to an inhibitory effect of lactoferrin - the production of granulocyte monocyte colony-stimulating activity - it was found that only the monomeric form of lactoferrin was inhibitory.

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Calcium-dependent polymerization of lactoferrin

Abstract

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