Calcium-dependent inactivation of synaptic NMDA receptors in hippocampal neurons

1. We examined whether synaptically activated N-methyl-D-aspartate (NMDA) receptors are regulated by intracellular calcium in cultured hippocampal neurons by comparing excitatory postsynaptic currents (EPSCs) to the previously described calcium-dependent regulation of whole cell NMDA currents. Standard whole cell recording and fast application methods were used. 2. Low-frequency (0.2 Hz) stimulation of EPSCs in the presence of 2- amino-5-phosphonovalerate (AP5) evoked a constant amplitude α-amino-3- hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor mediated EPSC. On removal of AP5 in Ca²⁺containing solutions, the amplitude of the slow NMDA receptor-mediated EPSC decreased by ~50% during the next 10 stimuli. The decrease in the EPSC was dependent on the extracellular calcium concentration and stimulus frequency, consistent with Ca²⁺-dependent desensitization/inactivation of postsynaptic NMDA receptors. A whole cell prepulse of NMDA (10 μM, 10 s) in Ca²⁺-containing solutions inhibited the slow EPSC to a similar degree. A series of slow EPSCs also produced Ca²⁺- dependent inactivation of whole cell NMDA currents evoked in low calcium solutions. 3. These results demonstrate that synaptic NMDA receptors are inactivated by intracellular calcium and that calcium entry through synaptically activated NMDA receptors is sufficient to provide feedback inhibition of the slow EPSC.